{"id":4193,"date":"2024-05-09T16:20:44","date_gmt":"2024-05-09T14:20:44","guid":{"rendered":"https:\/\/marte.i3a.es\/?p=4193"},"modified":"2025-06-30T12:27:40","modified_gmt":"2025-06-30T10:27:40","slug":"nanolyme-hacia-un-nuevo-diagnostico-de-la-enfermedad-de-lyme-a-traves-de-la-nanotecnologia-la-espectrometria-atomica-y-la-inteligencia-artificial","status":"publish","type":"post","link":"https:\/\/marte.i3a.es\/es\/nanolyme-hacia-un-nuevo-diagnostico-de-la-enfermedad-de-lyme-a-traves-de-la-nanotecnologia-la-espectrometria-atomica-y-la-inteligencia-artificial\/","title":{"rendered":"NanoLyme: Toward a new diagnosis of Lyme disease through nanotechnology, atomic spectrometry and artificial intelligence"},"content":{"rendered":"<p><style>\/*! elementor - v3.5.5 - 03-02-2022 *\/<br \/>\n@media (min-width:768px){.elementor-widget-icon-box.elementor-position-left .elementor-icon-box-wrapper,.elementor-widget-icon-box.elementor-position-right .elementor-icon-box-wrapper{display:-webkit-box;display:-ms-flexbox;display:flex}.elementor-widget-icon-box.elementor-position-left .elementor-icon-box-icon,.elementor-widget-icon-box.elementor-position-right .elementor-icon-box-icon{display:-webkit-inline-box;display:-ms-inline-flexbox;display:inline-flex;-webkit-box-flex:0;-ms-flex:0 0 auto;flex:0 0 auto}.elementor-widget-icon-box.elementor-position-right .elementor-icon-box-wrapper{text-align:right;-webkit-box-orient:horizontal;-webkit-box-direction:reverse;-ms-flex-direction:row-reverse;flex-direction:row-reverse}.elementor-widget-icon-box.elementor-position-left .elementor-icon-box-wrapper{text-align:left;-webkit-box-orient:horizontal;-webkit-box-direction:normal;-ms-flex-direction:row;flex-direction:row}.elementor-widget-icon-box.elementor-position-top .elementor-icon-box-img{margin:auto}.elementor-widget-icon-box.elementor-vertical-align-top .elementor-icon-box-wrapper{-webkit-box-align:start;-ms-flex-align:start;align-items:flex-start}.elementor-widget-icon-box.elementor-vertical-align-middle .elementor-icon-box-wrapper{-webkit-box-align:center;-ms-flex-align:center;align-items:center}.elementor-widget-icon-box.elementor-vertical-align-bottom .elementor-icon-box-wrapper{-webkit-box-align:end;-ms-flex-align:end;align-items:flex-end}}@media (max-width:767px){.elementor-widget-icon-box .elementor-icon-box-icon{margin-left:auto!important;margin-right:auto!important;margin-bottom:15px}}.elementor-widget-icon-box .elementor-icon-box-wrapper{text-align:center}.elementor-widget-icon-box .elementor-icon-box-title a{color:inherit}.elementor-widget-icon-box .elementor-icon-box-content{-webkit-box-flex:1;-ms-flex-positive:1;flex-grow:1}.elementor-widget-icon-box .elementor-icon-box-description{margin:0}<\/style><\/p>\n\n\n<div id=\"pl-gb4193-69d208a83e877\"  class=\"panel-layout wp-block-siteorigin-panels-layout-block\" ><div id=\"pg-gb4193-69d208a83e877-0\"  class=\"panel-grid panel-has-style\" ><div class=\"siteorigin-panels-stretch panel-row-style panel-row-style-for-gb4193-69d208a83e877-0\" data-stretch-type=\"full-width-stretch\" ><div id=\"pgc-gb4193-69d208a83e877-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4193-69d208a83e877-0-0-0\" class=\"so-panel widget widget_sow-hero panel-first-child panel-last-child\" data-index=\"0\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-hero so-widget-sow-hero-default-7a011a7c0fd5-4193 so-widget-fittext-wrapper\"\n\t\t\t data-fit-text-compressor=\"0.85\"\n\t\t>\t\t\t\t<div class=\"sow-slider-base\" style=\"display: none\" tabindex=\"0\">\n\t\t\t\t\t<ul\n\t\t\t\t\tclass=\"sow-slider-images\"\n\t\t\t\t\tdata-settings=\"{&quot;pagination&quot;:true,&quot;speed&quot;:800,&quot;timeout&quot;:8000,&quot;paused&quot;:false,&quot;pause_on_hover&quot;:false,&quot;swipe&quot;:true,&quot;nav_always_show_desktop&quot;:&quot;&quot;,&quot;nav_always_show_mobile&quot;:&quot;&quot;,&quot;breakpoint&quot;:&quot;780px&quot;,&quot;unmute&quot;:false,&quot;anchor&quot;:null}\"\n\t\t\t\t\t\t\t\t\t\tdata-anchor-id=\"\"\n\t\t\t\t>\t\t<li class=\"sow-slider-image\" style=\"visibility: visible;;background-color: #1e73be\" >\n\t\t\t\t\t<div class=\"sow-slider-image-container\">\n\t\t\t<div class=\"sow-slider-image-wrapper\">\n\t\t\t\t<h3 style=\"text-align: center\"><a href=\"\/es\/all-projects\/\">Proyectos<\/a><\/h3>\n<h1 class=\"entry-title\" style=\"text-align: center\"><strong>Interreg POCTEFA EFA 099\/01. NanoLyme: Hacia un nuevo diagn\u00f3stico de la enfermedad de Lyme a trav\u00e9s de la nanotecnolog\u00eda, la espectrometr\u00eda at\u00f3mica y la inteligencia artificial<\/strong><\/h1>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/li>\n\t\t<\/ul>\t\t\t\t<ol class=\"sow-slider-pagination\">\n\t\t\t\t\t\t\t\t\t\t\t<li><a href=\"#\" data-goto=\"0\" aria-label=\"mostrar diapositiva 1\"><\/a><\/li>\n\t\t\t\t\t\t\t\t\t<\/ol>\n\n\t\t\t\t<div class=\"sow-slide-nav sow-slide-nav-next\">\n\t\t\t\t\t<a href=\"#\" data-goto=\"next\" aria-label=\"diapositiva siguiente\" data-action=\"next\">\n\t\t\t\t\t\t<em class=\"sow-sld-icon-thin-right\"><\/em>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\n\t\t\t\t<div class=\"sow-slide-nav sow-slide-nav-prev\">\n\t\t\t\t\t<a href=\"#\" data-goto=\"previous\" aria-label=\"diapositiva anterior\" data-action=\"prev\">\n\t\t\t\t\t\t<em class=\"sow-sld-icon-thin-left\"><\/em>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div><\/div><\/div><\/div><\/div><\/div><\/div>\n\n<div id=\"pl-gb4193-69d208a83f52f\"  class=\"panel-layout wp-block-siteorigin-panels-layout-block\" ><div id=\"pg-gb4193-69d208a83f52f-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-gb4193-69d208a83f52f-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4193-69d208a83f52f-0-0-0\" class=\"so-panel widget widget_sow-image panel-first-child panel-last-child\" data-index=\"0\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-image so-widget-sow-image-default-8b5b6f678277-4193\"\n\t\t\t\n\t\t>\n<div class=\"sow-image-container\">\n\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/marte.i3a.es\/wp-content\/uploads\/2024\/05\/logo-nanolyme.png\" width=\"2342\" height=\"385\" srcset=\"https:\/\/marte.i3a.es\/wp-content\/uploads\/2024\/05\/logo-nanolyme.png 2342w, https:\/\/marte.i3a.es\/wp-content\/uploads\/2024\/05\/logo-nanolyme-300x49.png 300w, https:\/\/marte.i3a.es\/wp-content\/uploads\/2024\/05\/logo-nanolyme-1024x168.png 1024w, https:\/\/marte.i3a.es\/wp-content\/uploads\/2024\/05\/logo-nanolyme-768x126.png 768w, https:\/\/marte.i3a.es\/wp-content\/uploads\/2024\/05\/logo-nanolyme-1536x253.png 1536w, https:\/\/marte.i3a.es\/wp-content\/uploads\/2024\/05\/logo-nanolyme-2048x337.png 2048w, https:\/\/marte.i3a.es\/wp-content\/uploads\/2024\/05\/logo-nanolyme-18x3.png 18w\" sizes=\"auto, (max-width: 2342px) 100vw, 2342px\" title=\"logo nanolyme\" alt=\"\" \t\tclass=\"so-widget-image\"\/>\n\t<\/div>\n\n<\/div><\/div><\/div><div id=\"pgc-gb4193-69d208a83f52f-0-1\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4193-69d208a83f52f-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"panel-widget-style panel-widget-style-for-gb4193-69d208a83f52f-0-1-0\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<ul>\n<li><strong>Fecha de inicio<\/strong> 01-03-2024<\/li>\n<li><strong>Fecha de finalizaci\u00f3n<\/strong> 28-02-2027<\/li>\n<li><strong>Investigador Principal<\/strong>: Mart\u00edn Resano y Eduardo Bolea Fern\u00e1ndez<\/li>\n<li><strong>Tipo<\/strong>: Interreg VI-A Espa\u00f1a-Francia-Andorra (POCTEFA 2021-2027)<\/li>\n<li><strong>P\u00e1gina web<\/strong>: <a href=\"https:\/\/nanolyme.eu\/\">https:\/\/nanolyme.eu\/<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><div id=\"pg-gb4193-69d208a83f52f-1\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-gb4193-69d208a83f52f-1-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4193-69d208a83f52f-1-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"2\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p><strong>Resumen<\/strong>: La enfermedad de Lyme es una enfermedad emergente que est\u00e1 suscitando una preocupaci\u00f3n creciente a nivel cl\u00ednico en toda Europa. Se considera end\u00e9mica en las regiones POCTEFA y su incremento se debe, entre otros factores, al cambio clim\u00e1tico que hace que los vectores que la transmiten se encuentren activos durante m\u00e1s tiempo, tal y como recoge el informe \"El Cambio clim\u00e1tico es los Pirineos: impactos, vulnerabilidades y adaptaci\u00f3n\", elaborado por el OPCC y la CTP. La Resoluci\u00f3n del Parlamento Europeo sobre esta enfermedad, 2018\/2774 (RSP), analiza la situaci\u00f3n en Europa se\u00f1alando que \"un diagn\u00f3stico precoz m\u00e1s fiable de la enfermedad de Lyme reducir\u00e1 significativamente el n\u00famero de casos de dicha enfermedad en fase avanzada, mejor\u00e1ndose con ello la calidad de vida de los pacientes\" y pidiendo \"una mayor cooperaci\u00f3n internacional en la investigaci\u00f3n sobre la enfermedad de Lyme\", Nuestro proyecto recoge esta llamada capitalizando la experiencia previa adquirida en el diagn\u00f3stico de otra enfermedad (Wilson) en el proyecto previo DBS e incorporando al socio competente en Lyme en zona POCTEFA (CH Lannemezan). El proyecto persigue el desarrollo de un nuevo m\u00e9todo de diagn\u00f3stico de la enfermedad de Lyme a partir de un enfoque novedoso y \u00fanico, que en lugar de intentar detectar los anticuerpos generados por el paciente (a veces, pr\u00e1cticamente inexistentes), detectar\u00e1 las propias bacterias inoculadas que causan la enfermedad. Este m\u00e9todo s\u00f3lo se puede desarrollar a partir de la cooperaci\u00f3n transfronteriza de los socios participantes, y su experiencia complementaria en el uso de la nanotecnolog\u00eda como herramienta facilitadora, de la t\u00e9cnica ICP-MS y de la inteligencia artificial aplicada al diagn\u00f3stico cl\u00ednico. El resultado ser\u00e1 un m\u00e9todo robusto de diagn\u00f3stico de Lyme que ser\u00e1 directamente transferido a los principales hospitales de las regiones POCTEFA involucradas para su aplicaci\u00f3n inmediata al final del proyecto.<\/p>\n<p>El proyecto NanoLyme ha sido cofinanciado al 65% por la Uni\u00f3n Europea a trav\u00e9s del Programa Interreg VI-A Espa\u00f1a-Francia-Andorra (POCTEFA 2021-2027). El objetivo del POCTEFA es reforzar la integraci\u00f3n econ\u00f3mica y social de la zona fronteriza Espa\u00f1a-Francia-Andorra.<\/p>\n<p><iframe loading=\"lazy\" title=\"NanoLyme: La nanotecnolog\u00eda contra la enfermedad de Lyme\" width=\"1200\" height=\"675\" src=\"https:\/\/www.youtube.com\/embed\/KFD1hAMQp6s?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><div id=\"pg-gb4193-69d208a83f52f-2\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-gb4193-69d208a83f52f-2-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4193-69d208a83f52f-2-0-0\" class=\"so-panel widget widget_sow-headline panel-first-child\" data-index=\"3\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-headline so-widget-sow-headline-default-244eb6bef45a-4193\"\n\t\t\t\n\t\t><div class=\"sow-headline-container\">\n\t\t\t\t\t\t\t<h5 class=\"sow-headline\">\n\t\t\t\t\t\tPUBLICACIONES\t\t\t\t\t\t<\/h5>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"decoration\">\n\t\t\t\t\t\t<div class=\"decoration-inside\"><\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div><div id=\"panel-gb4193-69d208a83f52f-2-0-1\" class=\"so-panel widget widget_sow-editor panel-last-child\" data-index=\"4\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<div class=\"teachpress_pub_list\"><form name=\"tppublistform\" method=\"get\" action=\"\"><a name=\"tppubs\" id=\"tppubs\"><\/a><div class=\"teachpress_filter\"><select class=\"default\" name=\"yr\" id=\"yr\" tabindex=\"2\" onchange=\"teachpress_jumpMenu('parent',this, 'https:\/\/marte.i3a.es\/es\/nanolyme-hacia-un-nuevo-diagnostico-de-la-enfermedad-de-lyme-a-traves-de-la-nanotecnologia-la-espectrometria-atomica-y-la-inteligencia-artificial\/?')\">\r\n                   <option value=\"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=#tppubs\">Todos los a\u00f1os<\/option>\r\n                   <option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2025#tppubs\" >2025<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2024#tppubs\" >2024<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2023#tppubs\" >2023<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2022#tppubs\" >2022<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2021#tppubs\" >2021<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2020#tppubs\" >2020<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2019#tppubs\" >2019<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2018#tppubs\" >2018<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2017#tppubs\" >2017<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2016#tppubs\" >2016<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2015#tppubs\" >2015<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2014#tppubs\" >2014<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2013#tppubs\" >2013<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2012#tppubs\" >2012<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2004#tppubs\" >2004<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=0000#tppubs\" >0000<\/option>\r\n                <\/select><select class=\"default\" name=\"type\" id=\"type\" tabindex=\"3\" onchange=\"teachpress_jumpMenu('parent',this, 'https:\/\/marte.i3a.es\/es\/nanolyme-hacia-un-nuevo-diagnostico-de-la-enfermedad-de-lyme-a-traves-de-la-nanotecnologia-la-espectrometria-atomica-y-la-inteligencia-artificial\/?')\">\r\n                   <option value=\"tgid=&amp;yr=&amp;auth=&amp;usr=&amp;type=#tppubs\">Todas las tipolog\u00edas<\/option>\r\n                   <option value = \"tgid=&amp;yr=&amp;auth=&amp;usr=&amp;type=article#tppubs\" >Art\u00edculos de revista<\/option><option value = \"tgid=&amp;yr=&amp;auth=&amp;usr=&amp;type=proceedings#tppubs\" >Actas de congresos<\/option>\r\n                <\/select><\/div><input type=\"hidden\" name=\"trp-form-language\" value=\"es\"\/><\/form><div class=\"teachpress_publication_list\"><h3 class=\"tp_h3\" id=\"tp_h3_2025\">2025<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Rua-Ibarz, Ana;  Nakadi, Fl\u00e1vio V.;  Bolea-Fernandez, Eduardo;  Bazo, Antonio;  Battistella, Beatrice;  Matiushkina, Anna;  Resch-Genger, Ute;  Abad, Carlos;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('262','tp_links')\" style=\"cursor:pointer;\">Discrete Entity Analysis via Microwave-Induced Nitrogen Plasma\u2013Mass Spectrometry in Single-Event Mode<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 0, <\/span><span class=\"tp_pub_additional_number\">no 0, <\/span><span class=\"tp_pub_additional_pages\">pp. null, <\/span><span class=\"tp_pub_additional_year\">2025<\/span><span class=\"tp_pub_additional_note\">, (PMID: 41084806)<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('262','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('262','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('262','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_262\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{,<br \/>\r\ntitle = {Discrete Entity Analysis via Microwave-Induced Nitrogen Plasma\u2013Mass Spectrometry in Single-Event Mode},<br \/>\r\nauthor = {Ana Rua-Ibarz and Fl\u00e1vio V. Nakadi and Eduardo Bolea-Fernandez and Antonio Bazo and Beatrice Battistella and Anna Matiushkina and Ute Resch-Genger and Carlos Abad and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/doi.org\/10.1021\/acs.analchem.5c04341},<br \/>\r\ndoi = {10.1021\/acs.analchem.5c04341},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-10-14},<br \/>\r\nurldate = {2025-10-14},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {0},<br \/>\r\nnumber = {0},<br \/>\r\npages = {null},<br \/>\r\nabstract = {In this work, single-event microwave-induced nitrogen plasma\u2013mass spectrometry (single-event MINP-MS) was evaluated for the first time for the analysis of discrete entities such as nanoparticles, biological cells, and microplastics. Nitrogen (N2) effectively overcomes Ar-based polyatomic interferences, enabling (ultra)trace element determination of Fe and Se using their most abundant isotopes, 56Fe (91.66%) and 80Se (49.82%). Iron oxide nanoparticles (Fe2O3 NPs) ranging from 20 to 70 nm were accurately characterized, with excellent agreement with established sizing techniques, such as transmission electron microscopy (TEM) and dynamic light scattering (DLS). A limit of detection (LoD) of 8.6 ag for Fe\u2500equivalent to an LoDsize of 19 nm for Fe2O3\u2500was achieved, which is significantly lower than recent values reported for high-end quadrupole-based ICP-MS. Selenium nanoparticles (SeNPs) of 150 and 250 nm were also accurately characterized, without the N2-based plasma experiencing issues handling relatively large metallic NPs (linearity, R2 = 0.9994). Se-enriched yeast cells (SELM-1 certified reference material) were successfully analyzed via single-cell MINP-MS using external calibration based on SeNPs and a transport efficiency-independent approach. In addition, 2\u20133 \u03bcm polystyrene (PS) and polytetrafluoroethylene (PTFE) were accurately sized by monitoring 12C+, confirming the method\u2019s suitability for handling micrometer-sized polymeric materials (microplastics). The average duration of individual events (680 \u00b1 160 \u03bcs) suggests that the digestion of individual entities in N2-based plasmas is comparable to that in Ar-based plasmas. These results open new avenues for this instrumentation as an alternative to ICP ionization sources, also in the context of discrete entity analysis.},<br \/>\r\nnote = {PMID: 41084806},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('262','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_262\" style=\"display:none;\"><div class=\"tp_abstract_entry\">In this work, single-event microwave-induced nitrogen plasma\u2013mass spectrometry (single-event MINP-MS) was evaluated for the first time for the analysis of discrete entities such as nanoparticles, biological cells, and microplastics. Nitrogen (N2) effectively overcomes Ar-based polyatomic interferences, enabling (ultra)trace element determination of Fe and Se using their most abundant isotopes, 56Fe (91.66%) and 80Se (49.82%). Iron oxide nanoparticles (Fe2O3 NPs) ranging from 20 to 70 nm were accurately characterized, with excellent agreement with established sizing techniques, such as transmission electron microscopy (TEM) and dynamic light scattering (DLS). A limit of detection (LoD) of 8.6 ag for Fe\u2500equivalent to an LoDsize of 19 nm for Fe2O3\u2500was achieved, which is significantly lower than recent values reported for high-end quadrupole-based ICP-MS. Selenium nanoparticles (SeNPs) of 150 and 250 nm were also accurately characterized, without the N2-based plasma experiencing issues handling relatively large metallic NPs (linearity, R2 = 0.9994). Se-enriched yeast cells (SELM-1 certified reference material) were successfully analyzed via single-cell MINP-MS using external calibration based on SeNPs and a transport efficiency-independent approach. In addition, 2\u20133 \u03bcm polystyrene (PS) and polytetrafluoroethylene (PTFE) were accurately sized by monitoring 12C+, confirming the method\u2019s suitability for handling micrometer-sized polymeric materials (microplastics). The average duration of individual events (680 \u00b1 160 \u03bcs) suggests that the digestion of individual entities in N2-based plasmas is comparable to that in Ar-based plasmas. These results open new avenues for this instrumentation as an alternative to ICP ionization sources, also in the context of discrete entity analysis.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('262','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_262\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/doi.org\/10.1021\/acs.analchem.5c04341\" title=\"https:\/\/doi.org\/10.1021\/acs.analchem.5c04341\" target=\"_blank\">https:\/\/doi.org\/10.1021\/acs.analchem.5c04341<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.1021\/acs.analchem.5c04341\" title=\"DOI de seguimiento:10.1021\/acs.analchem.5c04341\" target=\"_blank\">doi:10.1021\/acs.analchem.5c04341<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('262','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Bazo, Antonio;  Bolea-Fernandez, Eduardo;  Rua-Ibarz, Ana;  Aramend\u00eda, Maite;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('258','tp_links')\" style=\"cursor:pointer;\">Ions with Ions, Entities with Entities: A Proof-of-Concept Study Using the SELM-1 Yeast Certified Reference Material for Intra- and Extracellular Se Quantification via Single-Cell ICP-Mass Spectrometry<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Anal. Chem., <\/span><span class=\"tp_pub_additional_year\">2025<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 1520-6882<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('258','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('258','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('258','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_258\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Ions with Ions, Entities with Entities: A Proof-of-Concept Study Using the SELM-1 Yeast Certified Reference Material for Intra- and Extracellular Se Quantification via Single-Cell ICP-Mass Spectrometry},<br \/>\r\nauthor = {Antonio Bazo and Eduardo Bolea-Fernandez and Ana Rua-Ibarz and Maite Aramend\u00eda and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c01588},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1021\/acs.analchem.5c01588},<br \/>\r\nissn = {1520-6882},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-06-07},<br \/>\r\nurldate = {2025-06-07},<br \/>\r\njournal = {Anal. Chem.},<br \/>\r\nabstract = {In this work, two novel nanoparticle (NP)-based calibration strategies, external calibration and a relative method, have been explored for single-cell ICP-mass spectrometry (SC-ICP-MS) analysis. The fundamental principle of these methods is to rely on individual entities (well-characterized NPs of the target analyte) for calibration rather than on ionic standard solutions. The performance of the NP-based calibration approaches has been compared to that of the reference method (particle size with AuNP standards). In addition to the intracellular Se content (mass per individual cell), the extracellular Se (dissolved fraction) was also determined directly and simultaneously using the average background from the SC-ICP-MS time-resolved signal. The figures-of-merit of the methods developed have been evaluated by relying on the analysis of the SELM-1 cell-certified reference material, consisting of Se-enriched yeast cells, and certified for its total Se content (intracellular + extracellular Se). All methods successfully determined the Se elemental contents, but an improvement in accuracy and precision was observed for the NP-based methods compared to the reference one. Furthermore, the NP-based methods were found to be less time-consuming, more straightforward, and more user-friendly in terms of calculations. These results open new avenues for calibration in quantitative SC-ICP-MS analysis and call for a fundamental change in the methodology, where the determination of ionic contents is based on the use of ionic standard solutions for calibration, while the determination of elemental contents in discrete micro\/nanoentities, such as cells, should ideally be based on calibration using standard entities, thus avoiding the need to calculate a transport efficiency coefficient.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('258','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_258\" style=\"display:none;\"><div class=\"tp_abstract_entry\">In this work, two novel nanoparticle (NP)-based calibration strategies, external calibration and a relative method, have been explored for single-cell ICP-mass spectrometry (SC-ICP-MS) analysis. The fundamental principle of these methods is to rely on individual entities (well-characterized NPs of the target analyte) for calibration rather than on ionic standard solutions. The performance of the NP-based calibration approaches has been compared to that of the reference method (particle size with AuNP standards). In addition to the intracellular Se content (mass per individual cell), the extracellular Se (dissolved fraction) was also determined directly and simultaneously using the average background from the SC-ICP-MS time-resolved signal. The figures-of-merit of the methods developed have been evaluated by relying on the analysis of the SELM-1 cell-certified reference material, consisting of Se-enriched yeast cells, and certified for its total Se content (intracellular + extracellular Se). All methods successfully determined the Se elemental contents, but an improvement in accuracy and precision was observed for the NP-based methods compared to the reference one. Furthermore, the NP-based methods were found to be less time-consuming, more straightforward, and more user-friendly in terms of calculations. These results open new avenues for calibration in quantitative SC-ICP-MS analysis and call for a fundamental change in the methodology, where the determination of ionic contents is based on the use of ionic standard solutions for calibration, while the determination of elemental contents in discrete micro\/nanoentities, such as cells, should ideally be based on calibration using standard entities, thus avoiding the need to calculate a transport efficiency coefficient.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('258','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_258\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c01588\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c01588\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c01588<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1021\/acs.analchem.5c01588\" title=\"DOI de seguimiento:https:\/\/doi.org\/10.1021\/acs.analchem.5c01588\" target=\"_blank\">doi:https:\/\/doi.org\/10.1021\/acs.analchem.5c01588<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('258','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Nakadi, Fl\u00e1vio V.;  Garcia-Garcia, Alicia;  Rua-Ibarz, Ana;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('254','tp_links')\" style=\"cursor:pointer;\">LAMIS in the gas phase: A new approach for obtaining Ca elemental and isotopic information via CaF molecule formation<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Talanta, <\/span><span class=\"tp_pub_additional_volume\">vol. 292, <\/span><span class=\"tp_pub_additional_pages\">pp. 127920, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 0039-9140<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('254','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('254','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('254','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_254\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{NAKADI2025127920,<br \/>\r\ntitle = {LAMIS in the gas phase: A new approach for obtaining Ca elemental and isotopic information via CaF molecule formation},<br \/>\r\nauthor = {Fl\u00e1vio V. Nakadi and Alicia Garcia-Garcia and Ana Rua-Ibarz and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0039914025004102},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.talanta.2025.127920},<br \/>\r\nissn = {0039-9140},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-01-01},<br \/>\r\nurldate = {2025-01-01},<br \/>\r\njournal = {Talanta},<br \/>\r\nvolume = {292},<br \/>\r\npages = {127920},<br \/>\r\nabstract = {This work introduces a novel method for generating the calcium monofluoride (CaF) diatomic molecule by adding the molecule-forming reagent in the gaseous phase (a methyl fluoride-argon mixture), in order to perform laser-induced breakdown spectroscopy (LIBS) and laser ablation molecular isotopic spectrometry (LAMIS) measurements. By optimizing the instrumental parameters, CaF molecule formation was successfully achieved within the plasma plume, upon ablation of dried liquid samples. The isotopic shift for the X2\u03a3\u2192A2\u03a0 (0,1) CaF vibronic transition at 583.0\u00a0nm was calculated to be 292.3 pm. The method proved capable of providing quantitative information for determining calcium concentrations in real samples, such as tap water and skimmed milk, using internal standardization (with Sr as internal standard; limit of detection, LOD, 20\u00a0mg\u00a0L\u22121) and isotope dilution (which can be applied from 400\u00a0mg\u00a0L\u22121on), respectively. Partial least squares regression (PLS) analysis was employed to enhance the quality of the isotopic data. The Ca concentration found in the tap water was 47\u00a0\u00b1\u00a016\u00a0mg\u00a0L\u22121 (reference flame atomic absorption spectrometry, FAAS, value: 59\u00a0\u00b1\u00a00.2\u00a0mg\u00a0L\u22121), and 1100\u00a0\u00b1\u00a0140\u00a0mg\u00a0L\u22121 for the skimmed milk (reference FAAS value: 1240\u00a0\u00b1\u00a0120\u00a0mg\u00a0L\u22121). No significant difference between LIBS and FAAS results could be established using a t-test at the 95% confidence level. Overall, this novel approach allows for the determination of calcium in terms of both the elemental concentration and the isotopic composition, thus broadening the applicability of LIBS (e.g., for tracer experiments, besides the already mentioned application of isotope dilution).},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('254','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_254\" style=\"display:none;\"><div class=\"tp_abstract_entry\">This work introduces a novel method for generating the calcium monofluoride (CaF) diatomic molecule by adding the molecule-forming reagent in the gaseous phase (a methyl fluoride-argon mixture), in order to perform laser-induced breakdown spectroscopy (LIBS) and laser ablation molecular isotopic spectrometry (LAMIS) measurements. By optimizing the instrumental parameters, CaF molecule formation was successfully achieved within the plasma plume, upon ablation of dried liquid samples. The isotopic shift for the X2\u03a3\u2192A2\u03a0 (0,1) CaF vibronic transition at 583.0\u00a0nm was calculated to be 292.3 pm. The method proved capable of providing quantitative information for determining calcium concentrations in real samples, such as tap water and skimmed milk, using internal standardization (with Sr as internal standard; limit of detection, LOD, 20\u00a0mg\u00a0L\u22121) and isotope dilution (which can be applied from 400\u00a0mg\u00a0L\u22121on), respectively. Partial least squares regression (PLS) analysis was employed to enhance the quality of the isotopic data. The Ca concentration found in the tap water was 47\u00a0\u00b1\u00a016\u00a0mg\u00a0L\u22121 (reference flame atomic absorption spectrometry, FAAS, value: 59\u00a0\u00b1\u00a00.2\u00a0mg\u00a0L\u22121), and 1100\u00a0\u00b1\u00a0140\u00a0mg\u00a0L\u22121 for the skimmed milk (reference FAAS value: 1240\u00a0\u00b1\u00a0120\u00a0mg\u00a0L\u22121). No significant difference between LIBS and FAAS results could be established using a t-test at the 95% confidence level. Overall, this novel approach allows for the determination of calcium in terms of both the elemental concentration and the isotopic composition, thus broadening the applicability of LIBS (e.g., for tracer experiments, besides the already mentioned application of isotope dilution).<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('254','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_254\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0039914025004102\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0039914025004102\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0039914025004102<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.talanta.2025.127920\" title=\"DOI de seguimiento:https:\/\/doi.org\/10.1016\/j.talanta.2025.127920\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.talanta.2025.127920<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('254','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Bazo, Antonio;  Bolea-Fernandez, Eduardo;  Billimoria, Kharmen;  Rua-Ibarz, Ana;  Aramend\u00eda, Maite;  Menero-Vald\u00e9s, Paula;  Morley, Jack;  Neves, Sara;  S\u00e1nchez-Cachero, Armando;  Goenaga-Infante, Heidi;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('259','tp_links')\" style=\"cursor:pointer;\">A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">J. Anal. At. Spectrom., <\/span><span class=\"tp_pub_additional_pages\">pp. -, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_259\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('259','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_259\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('259','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_259\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('259','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_259\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{D5JA00253B,<br \/>\r\ntitle = {A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study},<br \/>\r\nauthor = {Antonio Bazo and Eduardo Bolea-Fernandez and Kharmen Billimoria and Ana Rua-Ibarz and Maite Aramend\u00eda and Paula Menero-Vald\u00e9s and Jack Morley and Sara Neves and Armando S\u00e1nchez-Cachero and Heidi Goenaga-Infante and Mart\u00edn Resano},<br \/>\r\nurl = {http:\/\/dx.doi.org\/10.1039\/D5JA00253B},<br \/>\r\ndoi = {10.1039\/D5JA00253B},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-01-01},<br \/>\r\nurldate = {2025-01-01},<br \/>\r\njournal = {J. Anal. At. Spectrom.},<br \/>\r\npages = {-},<br \/>\r\npublisher = {The Royal Society of Chemistry},<br \/>\r\nabstract = {Laser ablation ICP-mass spectrometry (LA-ICP-MS) has developed as a powerful tool for elemental quantitative analysis of individual cells, assuring that the content of each cell is analyzed individually. However, this technique is still limited by the difficulties associated with calibration using solid standards. This work proposes a particle mass calibration strategy that is independent of both the properties and thickness of the gelatin films used for calibration, overcoming a significant drawback of previously established methods. The fundamental principle of this strategy relies on the individual ablation of nanoparticles (NPs) of well-characterized size that are embedded in the films, so that their mass can be directly used for calibration without the need to calculate their exact concentration within the gelatin. The performance of the newly developed method was compared to that of the previously reported approaches (ionic and particle number calibration) in terms of linearity and homogeneity between different films prepared from the same gelatin solution. As a case study, the three calibration strategies were used for the quantitative analysis of HeLa cancer cells exposed to AuNPs. In parallel, in-suspension single-cell (SC) ICP-MS Au data were obtained and used as reference for comparison with the three LA-SC-ICP-MS strategies. The results obtained with the novel particle mass approach demonstrated better accuracy and repeatability over three different working sessions, addressing key limitations and providing a robust and reliable method for quantitative LA-SC-ICP-MS analysis. The particle mass method holds promise for quantitative LA-ICP-MS analysis of samples beyond NP-exposed cells, such as biological tissues.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('259','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_259\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Laser ablation ICP-mass spectrometry (LA-ICP-MS) has developed as a powerful tool for elemental quantitative analysis of individual cells, assuring that the content of each cell is analyzed individually. However, this technique is still limited by the difficulties associated with calibration using solid standards. This work proposes a particle mass calibration strategy that is independent of both the properties and thickness of the gelatin films used for calibration, overcoming a significant drawback of previously established methods. The fundamental principle of this strategy relies on the individual ablation of nanoparticles (NPs) of well-characterized size that are embedded in the films, so that their mass can be directly used for calibration without the need to calculate their exact concentration within the gelatin. The performance of the newly developed method was compared to that of the previously reported approaches (ionic and particle number calibration) in terms of linearity and homogeneity between different films prepared from the same gelatin solution. As a case study, the three calibration strategies were used for the quantitative analysis of HeLa cancer cells exposed to AuNPs. In parallel, in-suspension single-cell (SC) ICP-MS Au data were obtained and used as reference for comparison with the three LA-SC-ICP-MS strategies. The results obtained with the novel particle mass approach demonstrated better accuracy and repeatability over three different working sessions, addressing key limitations and providing a robust and reliable method for quantitative LA-SC-ICP-MS analysis. The particle mass method holds promise for quantitative LA-ICP-MS analysis of samples beyond NP-exposed cells, such as biological tissues.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('259','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_259\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"http:\/\/dx.doi.org\/10.1039\/D5JA00253B\" title=\"http:\/\/dx.doi.org\/10.1039\/D5JA00253B\" target=\"_blank\">http:\/\/dx.doi.org\/10.1039\/D5JA00253B<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.1039\/D5JA00253B\" title=\"DOI de seguimiento:10.1039\/D5JA00253B\" target=\"_blank\">doi:10.1039\/D5JA00253B<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('259','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Bazo, Antonio;  L\u00f3pez-Villellas, Lori\u00e9n;  Mataloni, Matilde;  Bolea-Fernandez, Eduardo;  Rua-Ibarz, Ana;  Grotti, Marco;  Aramend\u00eda, Maite;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('260','tp_links')\" style=\"cursor:pointer;\">Improving detection and figures of merit in single-particle inductively coupled plasma-mass spectrometry via transient event heights<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Analytica Chimica Acta, <\/span><span class=\"tp_pub_additional_volume\">vol. 1378, <\/span><span class=\"tp_pub_additional_pages\">pp. 344694, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 0003-2670<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('260','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('260','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('260','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_260\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{BAZO2025344694,<br \/>\r\ntitle = {Improving detection and figures of merit in single-particle inductively coupled plasma-mass spectrometry via transient event heights},<br \/>\r\nauthor = {Antonio Bazo and Lori\u00e9n L\u00f3pez-Villellas and Matilde Mataloni and Eduardo Bolea-Fernandez and Ana Rua-Ibarz and Marco Grotti and Maite Aramend\u00eda and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267025010888},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.aca.2025.344694},<br \/>\r\nissn = {0003-2670},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-01-01},<br \/>\r\nurldate = {2025-01-01},<br \/>\r\njournal = {Analytica Chimica Acta},<br \/>\r\nvolume = {1378},<br \/>\r\npages = {344694},<br \/>\r\nabstract = {Background <br \/>\r\nSingle-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) is a powerful method for characterizing micro- and nanoparticulate materials. The technique primarily relies on the linear relationship between the integrated intensities of individual events (peak areas) and the analyte mass, though transit times (peak widths) have also been used for quantitative purposes. This work (1) evaluates the potential of using peak heights as analytical signals in SP-ICP-MS, (2) introduces a new method for determining peak heights, and (3) explores scenarios in which peak height offers added value over the commonly used SP-ICP-MS signals. <br \/>\r\nResults <br \/>\r\nA new method was proposed to estimate peak height values in SP-ICP-MS accurately. The cumulative intensity across consecutive dwell times was modeled using a third-degree polynomial, from which the adjusted peak height was derived. This approach reduces the uncertainty associated with using raw maximum intensity values, yielding NP distributions comparable to those obtained via integrated intensities. The effect of dwell time on peak height was also evaluated. An optimal range (50 \u03bcs\u2013200 \u03bcs) was identified, where a linear relationship was observed between the peak height and the square of the NP diameter. Within this range, peak height showed the lowest bias when characterizing smaller NPs, indicating the potential to improve the limit of quantification (LoQ). Additionally, peak heights proved helpful in determining the limit of detection (LoD) and setting appropriate threshold values for data processing, thereby helping to flag incorrect resultsand addressing a challenge in SP-ICP-MS analysis. <br \/>\r\nSignificance <br \/>\r\nThis is the first study to evaluate peak height as an analytical signal in SP-ICP-MS. The results highlight its advantages in specific applications, such as sizing NPs near the LoD, and in supporting the more reliable use of other signals, such as peak areas, by helping to identify incorrect threshold selection that could lead to biased distributions. Finally, monitoring peak heights allows for a more realistic and assumption-free determination of the LoD.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('260','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_260\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Background <br \/>\r\nSingle-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) is a powerful method for characterizing micro- and nanoparticulate materials. The technique primarily relies on the linear relationship between the integrated intensities of individual events (peak areas) and the analyte mass, though transit times (peak widths) have also been used for quantitative purposes. This work (1) evaluates the potential of using peak heights as analytical signals in SP-ICP-MS, (2) introduces a new method for determining peak heights, and (3) explores scenarios in which peak height offers added value over the commonly used SP-ICP-MS signals. <br \/>\r\nResults <br \/>\r\nA new method was proposed to estimate peak height values in SP-ICP-MS accurately. The cumulative intensity across consecutive dwell times was modeled using a third-degree polynomial, from which the adjusted peak height was derived. This approach reduces the uncertainty associated with using raw maximum intensity values, yielding NP distributions comparable to those obtained via integrated intensities. The effect of dwell time on peak height was also evaluated. An optimal range (50 \u03bcs\u2013200 \u03bcs) was identified, where a linear relationship was observed between the peak height and the square of the NP diameter. Within this range, peak height showed the lowest bias when characterizing smaller NPs, indicating the potential to improve the limit of quantification (LoQ). Additionally, peak heights proved helpful in determining the limit of detection (LoD) and setting appropriate threshold values for data processing, thereby helping to flag incorrect resultsand addressing a challenge in SP-ICP-MS analysis. <br \/>\r\nSignificance <br \/>\r\nThis is the first study to evaluate peak height as an analytical signal in SP-ICP-MS. The results highlight its advantages in specific applications, such as sizing NPs near the LoD, and in supporting the more reliable use of other signals, such as peak areas, by helping to identify incorrect threshold selection that could lead to biased distributions. Finally, monitoring peak heights allows for a more realistic and assumption-free determination of the LoD.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('260','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_260\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267025010888\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267025010888\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267025010888<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.aca.2025.344694\" title=\"DOI de seguimiento:https:\/\/doi.org\/10.1016\/j.aca.2025.344694\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.aca.2025.344694<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('260','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><h3 class=\"tp_h3\" id=\"tp_h3_2024\">2024<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Bazo, Antonio;  Bolea-Fernandez, Eduardo;  Rua-Ibarz, Ana;  Aramend\u00eda, Maite;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('240','tp_links')\" style=\"cursor:pointer;\">Intensity- and time-based strategies for micro\/nano-sizing via single-particle ICP-mass spectrometry: A comparative assessment using Au and SiO2 as model particles<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Analytica Chimica Acta, <\/span><span class=\"tp_pub_additional_volume\">vol. 1331, <\/span><span class=\"tp_pub_additional_pages\">pp. 343305, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 0003-2670<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_240\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('240','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_240\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('240','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_240\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('240','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_240\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{BAZO2024343305,<br \/>\r\ntitle = {Intensity- and time-based strategies for micro\/nano-sizing via single-particle ICP-mass spectrometry: A comparative assessment using Au and SiO2 as model particles},<br \/>\r\nauthor = {Antonio Bazo and Eduardo Bolea-Fernandez and Ana Rua-Ibarz and Maite Aramend\u00eda and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024011061},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.aca.2024.343305},<br \/>\r\nissn = {0003-2670},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-01-01},<br \/>\r\nurldate = {2024-01-01},<br \/>\r\njournal = {Analytica Chimica Acta},<br \/>\r\nvolume = {1331},<br \/>\r\npages = {343305},<br \/>\r\nabstract = {Background <br \/>\r\nSingle-particle ICP-mass spectrometry (SP-ICP-MS) is a powerful method for micro\/nano-particle (MNP) sizing. Despite the outstanding evolution of the technique in the last decade, most studies still rely on traditional approaches based on (1) the use of integrated intensity as the analytical signal and (2) the calculation of the transport efficiency (TE). However, the increasing availability of MNP standards and advancements in hardware and software have unveiled new venues for MNP sizing, including TE-independent and time-based approaches. This work systematically examines these different methodologies to identify and summarize their strengths and weaknesses, thus helping to determine their preferred application areas. <br \/>\r\nResults <br \/>\r\nDifferent SP-ICP-MS methods for MNP sizing were assessed using AuNPs (20\u201370\u00a0nm) and SiO2MNPs (100\u20131000\u00a0nm). Among TE-dependent approaches, the particle frequency method was characterized by larger uncertainties than the particle size method. The results of the latter were dependent on the appropriate selection of the reference MNP, making the use of multiple reference MNPs recommended. TE-independent methods were based on external (linear and polynomial) calibrations and a relative approach. These methods exhibited the lowest uncertainties of all the strategies evaluated. External calibrations benefited from simpler calculations, but their application could be hindered by a lack of reference MNPs within the desired size range or by the need for interpolations outside the calibration range. Finally, transit time signals are directly proportional to the MNP size rather than its mass. The time-based method demonstrated adequate performance for sizing AuNPs but failed when sizing the largest SiO2MNPs (1000\u00a0nm). <br \/>\r\nSignificance and novelty <br \/>\r\nThis work provides further insights into the application of different SP-ICP-MS methodologies for MNP sizing. Both TE-independent approaches and the monitoring of the transit time as the analytical signal are underused strategies; in this context, a Python script was developed for accurate transit time measurement. After 20 years of development, a quantitative comparison of the different methodologies, including the most novel approaches, is deemed necessary for further growth on solid theoretical ground.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('240','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_240\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Background <br \/>\r\nSingle-particle ICP-mass spectrometry (SP-ICP-MS) is a powerful method for micro\/nano-particle (MNP) sizing. Despite the outstanding evolution of the technique in the last decade, most studies still rely on traditional approaches based on (1) the use of integrated intensity as the analytical signal and (2) the calculation of the transport efficiency (TE). However, the increasing availability of MNP standards and advancements in hardware and software have unveiled new venues for MNP sizing, including TE-independent and time-based approaches. This work systematically examines these different methodologies to identify and summarize their strengths and weaknesses, thus helping to determine their preferred application areas. <br \/>\r\nResults <br \/>\r\nDifferent SP-ICP-MS methods for MNP sizing were assessed using AuNPs (20\u201370\u00a0nm) and SiO2MNPs (100\u20131000\u00a0nm). Among TE-dependent approaches, the particle frequency method was characterized by larger uncertainties than the particle size method. The results of the latter were dependent on the appropriate selection of the reference MNP, making the use of multiple reference MNPs recommended. TE-independent methods were based on external (linear and polynomial) calibrations and a relative approach. These methods exhibited the lowest uncertainties of all the strategies evaluated. External calibrations benefited from simpler calculations, but their application could be hindered by a lack of reference MNPs within the desired size range or by the need for interpolations outside the calibration range. Finally, transit time signals are directly proportional to the MNP size rather than its mass. The time-based method demonstrated adequate performance for sizing AuNPs but failed when sizing the largest SiO2MNPs (1000\u00a0nm). <br \/>\r\nSignificance and novelty <br \/>\r\nThis work provides further insights into the application of different SP-ICP-MS methodologies for MNP sizing. Both TE-independent approaches and the monitoring of the transit time as the analytical signal are underused strategies; in this context, a Python script was developed for accurate transit time measurement. After 20 years of development, a quantitative comparison of the different methodologies, including the most novel approaches, is deemed necessary for further growth on solid theoretical ground.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('240','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_240\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024011061\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024011061\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024011061<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.aca.2024.343305\" title=\"DOI de seguimiento:https:\/\/doi.org\/10.1016\/j.aca.2024.343305\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.aca.2024.343305<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('240','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><\/div><\/div>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":3,"featured_media":4289,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[226,230],"tags":[],"class_list":["post-4193","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-featured-projects","category-projects"],"_links":{"self":[{"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/posts\/4193","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/comments?post=4193"}],"version-history":[{"count":18,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/posts\/4193\/revisions"}],"predecessor-version":[{"id":4399,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/posts\/4193\/revisions\/4399"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/media\/4289"}],"wp:attachment":[{"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/media?parent=4193"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/categories?post=4193"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/tags?post=4193"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}