{"id":4278,"date":"2025-04-10T17:07:05","date_gmt":"2025-04-10T15:07:05","guid":{"rendered":"https:\/\/marte.i3a.es\/?p=4278"},"modified":"2025-06-30T13:30:37","modified_gmt":"2025-06-30T11:30:37","slug":"micromuestreo-y-alta-resolucion-temporal-y-espacial-para-el-analisis-clinico-minimamente-invasivo-y-el-analisis-de-celulas-individuales","status":"publish","type":"post","link":"https:\/\/marte.i3a.es\/es\/micromuestreo-y-alta-resolucion-temporal-y-espacial-para-el-analisis-clinico-minimamente-invasivo-y-el-analisis-de-celulas-individuales\/","title":{"rendered":"Microsampling and high-temporal and -spatial resolution for minimally invasive clinical analysis and single-cell analysis"},"content":{"rendered":"<p><style>\/*! elementor - v3.5.5 - 03-02-2022 *\/<br \/>\n@media (min-width:768px){.elementor-widget-icon-box.elementor-position-left .elementor-icon-box-wrapper,.elementor-widget-icon-box.elementor-position-right .elementor-icon-box-wrapper{display:-webkit-box;display:-ms-flexbox;display:flex}.elementor-widget-icon-box.elementor-position-left .elementor-icon-box-icon,.elementor-widget-icon-box.elementor-position-right .elementor-icon-box-icon{display:-webkit-inline-box;display:-ms-inline-flexbox;display:inline-flex;-webkit-box-flex:0;-ms-flex:0 0 auto;flex:0 0 auto}.elementor-widget-icon-box.elementor-position-right .elementor-icon-box-wrapper{text-align:right;-webkit-box-orient:horizontal;-webkit-box-direction:reverse;-ms-flex-direction:row-reverse;flex-direction:row-reverse}.elementor-widget-icon-box.elementor-position-left .elementor-icon-box-wrapper{text-align:left;-webkit-box-orient:horizontal;-webkit-box-direction:normal;-ms-flex-direction:row;flex-direction:row}.elementor-widget-icon-box.elementor-position-top .elementor-icon-box-img{margin:auto}.elementor-widget-icon-box.elementor-vertical-align-top .elementor-icon-box-wrapper{-webkit-box-align:start;-ms-flex-align:start;align-items:flex-start}.elementor-widget-icon-box.elementor-vertical-align-middle .elementor-icon-box-wrapper{-webkit-box-align:center;-ms-flex-align:center;align-items:center}.elementor-widget-icon-box.elementor-vertical-align-bottom .elementor-icon-box-wrapper{-webkit-box-align:end;-ms-flex-align:end;align-items:flex-end}}@media (max-width:767px){.elementor-widget-icon-box .elementor-icon-box-icon{margin-left:auto!important;margin-right:auto!important;margin-bottom:15px}}.elementor-widget-icon-box .elementor-icon-box-wrapper{text-align:center}.elementor-widget-icon-box .elementor-icon-box-title a{color:inherit}.elementor-widget-icon-box .elementor-icon-box-content{-webkit-box-flex:1;-ms-flex-positive:1;flex-grow:1}.elementor-widget-icon-box .elementor-icon-box-description{margin:0}<\/style><\/p>\n\n\n<div id=\"pl-gb4278-69ed738836703\"  class=\"panel-layout wp-block-siteorigin-panels-layout-block\" ><div id=\"pg-gb4278-69ed738836703-0\"  class=\"panel-grid panel-has-style\" ><div class=\"siteorigin-panels-stretch panel-row-style panel-row-style-for-gb4278-69ed738836703-0\" data-stretch-type=\"full-width-stretch\" ><div id=\"pgc-gb4278-69ed738836703-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4278-69ed738836703-0-0-0\" class=\"so-panel widget widget_sow-hero panel-first-child panel-last-child\" data-index=\"0\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-hero so-widget-sow-hero-default-7a011a7c0fd5-4278 so-widget-fittext-wrapper\"\n\t\t\t data-fit-text-compressor=\"0.85\"\n\t\t>\t\t\t\t<div class=\"sow-slider-base\" style=\"display: none\" tabindex=\"0\">\n\t\t\t\t\t<ul\n\t\t\t\t\tclass=\"sow-slider-images\"\n\t\t\t\t\tdata-settings=\"{&quot;pagination&quot;:true,&quot;speed&quot;:800,&quot;timeout&quot;:8000,&quot;paused&quot;:false,&quot;pause_on_hover&quot;:false,&quot;swipe&quot;:true,&quot;nav_always_show_desktop&quot;:&quot;&quot;,&quot;nav_always_show_mobile&quot;:&quot;&quot;,&quot;breakpoint&quot;:&quot;780px&quot;,&quot;unmute&quot;:false,&quot;anchor&quot;:null}\"\n\t\t\t\t\t\t\t\t\t\tdata-anchor-id=\"\"\n\t\t\t\t>\t\t<li class=\"sow-slider-image\" style=\"visibility: visible;;background-color: #1e73be\" >\n\t\t\t\t\t<div class=\"sow-slider-image-container\">\n\t\t\t<div class=\"sow-slider-image-wrapper\">\n\t\t\t\t<h3 style=\"text-align: center\"><a href=\"\/es\/all-projects\/\">Proyectos<\/a><\/h3>\n<h1 class=\"entry-title\" style=\"text-align: center\"><strong>PROY_E17_24. Micromuestreo y alta resoluci\u00f3n temporal y espacial para el an\u00e1lisis cl\u00ednico m\u00ednimamente invasivo y el an\u00e1lisis de c\u00e9lulas individuales<\/strong><\/h1>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/li>\n\t\t<\/ul>\t\t\t\t<ol class=\"sow-slider-pagination\">\n\t\t\t\t\t\t\t\t\t\t\t<li><a href=\"#\" data-goto=\"0\" aria-label=\"mostrar diapositiva 1\"><\/a><\/li>\n\t\t\t\t\t\t\t\t\t<\/ol>\n\n\t\t\t\t<div class=\"sow-slide-nav sow-slide-nav-next\">\n\t\t\t\t\t<a href=\"#\" data-goto=\"next\" aria-label=\"diapositiva siguiente\" data-action=\"next\">\n\t\t\t\t\t\t<em class=\"sow-sld-icon-thin-right\"><\/em>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\n\t\t\t\t<div class=\"sow-slide-nav sow-slide-nav-prev\">\n\t\t\t\t\t<a href=\"#\" data-goto=\"previous\" aria-label=\"diapositiva anterior\" data-action=\"prev\">\n\t\t\t\t\t\t<em class=\"sow-sld-icon-thin-left\"><\/em>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div><\/div><\/div><\/div><\/div><\/div><\/div>\n\n<div id=\"pl-gb4278-69ed7388373e0\"  class=\"panel-layout wp-block-siteorigin-panels-layout-block\" ><div id=\"pg-gb4278-69ed7388373e0-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-gb4278-69ed7388373e0-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4278-69ed7388373e0-0-0-0\" class=\"so-panel widget widget_sow-image panel-first-child panel-last-child\" data-index=\"0\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-image so-widget-sow-image-default-8b5b6f678277-4278\"\n\t\t\t\n\t\t>\n<div class=\"sow-image-container\">\n\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/marte.i3a.es\/wp-content\/uploads\/2025\/01\/Logotipo_DGA_Tecnologia.jpg\" width=\"425\" height=\"161\" srcset=\"https:\/\/marte.i3a.es\/wp-content\/uploads\/2025\/01\/Logotipo_DGA_Tecnologia.jpg 425w, https:\/\/marte.i3a.es\/wp-content\/uploads\/2025\/01\/Logotipo_DGA_Tecnologia-300x114.jpg 300w, https:\/\/marte.i3a.es\/wp-content\/uploads\/2025\/01\/Logotipo_DGA_Tecnologia-18x7.jpg 18w\" sizes=\"auto, (max-width: 425px) 100vw, 425px\" alt=\"\" \t\tclass=\"so-widget-image\"\/>\n\t<\/div>\n\n<\/div><\/div><\/div><div id=\"pgc-gb4278-69ed7388373e0-0-1\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4278-69ed7388373e0-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" ><div class=\"panel-widget-style panel-widget-style-for-gb4278-69ed7388373e0-0-1-0\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<ul>\n<li><strong>Fecha de inicio<\/strong> 01-01-2024<\/li>\n<li><strong>Fecha de finalizaci\u00f3n<\/strong> 31-12-2026<\/li>\n<li><strong>Investigador Principal<\/strong>: Mart\u00edn Resano y Eduardo Bolea Fern\u00e1ndez<\/li>\n<li><strong>Tipo<\/strong>: Desarrollo de proyectos de I+D+i en l\u00edneas prioritarias y de car\u00e1cter multidisciplinar para el periodo 2024-2026. Diputaci\u00f3n General de Arag\u00f3n<\/li>\n<\/ul>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><div id=\"pg-gb4278-69ed7388373e0-1\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-gb4278-69ed7388373e0-1-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4278-69ed7388373e0-1-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"2\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p><strong>Resumen<\/strong>: El proyecto persigue el desarrollo de nuevas metodolog\u00edas anal\u00edticas que permitan por un lado el an\u00e1lisis cuantitativo de dried blood spots y, por otro, el de c\u00e9lulas individuales, en ambos casos mediante la t\u00e9cnica de ICP-MS y usando nuevas estrategias de micromuestreo. Para conseguir mejorar estos m\u00e9todos y extender su rango de aplicaci\u00f3n es preciso explorar tanto el empleo de dispositivos novedosos que permitan la manipulaci\u00f3n de muestras en el rango de los micro y nanolitros, como el desarrollo de enfoques alternativos de introducci\u00f3n de muestras basadas en la microflu\u00eddica.<\/p>\n<p>El empleo de un sistema dispensador automatizado de nanol\u00edquidos puede ayudar al desarrollo de nuevos m\u00e9todos anal\u00edticos para la determinaci\u00f3n de bajas concentraciones de analitos en todo tipo de muestras micro\/nano, incluidas las c\u00e9lulas. Estos dispositivos son capaces de dispensar vol\u00famenes controlados y asegurar la presencia de c\u00e9lulas en cada uno de ellos con alta eficiencia, lo que facilita mucho el an\u00e1lisis de c\u00e9lulas individuales. Asimismo, estos dispositivos permiten la deposici\u00f3n precisa de nanovol\u00famenes de sangre u otros fluidos sobre soportes DBS, garantizando una distribuci\u00f3n m\u00e1s uniforme y controlada de la muestra. Empleando estos dispositivos, los DBS tambi\u00e9n pueden funcionalizarse con enzimas, marcadores o anticuerpos, lo que facilita su posterior an\u00e1lisis y abre el campo a la determinaci\u00f3n de otras especies m\u00e1s all\u00e1 de las elementales.<\/p>\n<p>El empleo de chips microflu\u00eddicos como medio de introducci\u00f3n de muestras puede considerarse un enfoque elegante para superar el problema de la introducci\u00f3n de muestras de una sola entidad. Estos chips ofrecen la posibilidad de encapsular c\u00e9lulas antes de introducirlas en el ICP, aumentando as\u00ed las posibilidades de que las c\u00e9lulas resistan al proceso de introducci\u00f3n como entidades intactas. Tambi\u00e9n es esperable que las eficiencias de introducci\u00f3n sean mucho mayores que las comparadas con otros enfoques alternativos. Finalmente, la funcionalizaci\u00f3n de estos dispositivos permite realizar operaciones adicionales, incluidas reacciones y separaciones. De esta manera se aportan nuevas v\u00edas para el an\u00e1lisis en l\u00ednea de diversos tipos de c\u00e9lulas y\/o para el marcaje con anticuerpos espec\u00edficos, lo que puede permitir estudiar diferentes tipos de c\u00e9lulas, estados y funciones en suspensiones celulares.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><div id=\"pg-gb4278-69ed7388373e0-2\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-gb4278-69ed7388373e0-2-0\"  class=\"panel-grid-cell\" ><div id=\"panel-gb4278-69ed7388373e0-2-0-0\" class=\"so-panel widget widget_sow-headline panel-first-child\" data-index=\"3\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-headline so-widget-sow-headline-default-244eb6bef45a-4278\"\n\t\t\t\n\t\t><div class=\"sow-headline-container\">\n\t\t\t\t\t\t\t<h5 class=\"sow-headline\">\n\t\t\t\t\t\tPUBLICACIONES\t\t\t\t\t\t<\/h5>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"decoration\">\n\t\t\t\t\t\t<div class=\"decoration-inside\"><\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div><div id=\"panel-gb4278-69ed7388373e0-2-0-1\" class=\"so-panel widget widget_sow-editor panel-last-child\" data-index=\"4\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<div class=\"teachpress_pub_list\"><form name=\"tppublistform\" method=\"get\" action=\"\"><a name=\"tppubs\" id=\"tppubs\"><\/a><div class=\"teachpress_filter\"><select class=\"default\" name=\"yr\" id=\"yr\" tabindex=\"2\" onchange=\"teachpress_jumpMenu('parent',this, 'https:\/\/marte.i3a.es\/es\/micromuestreo-y-alta-resolucion-temporal-y-espacial-para-el-analisis-clinico-minimamente-invasivo-y-el-analisis-de-celulas-individuales\/?')\">\r\n                   <option value=\"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=#tppubs\">Todos los a\u00f1os<\/option>\r\n                   <option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2025#tppubs\" >2025<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2024#tppubs\" >2024<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2023#tppubs\" >2023<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2022#tppubs\" >2022<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2021#tppubs\" >2021<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2020#tppubs\" >2020<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2019#tppubs\" >2019<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2018#tppubs\" >2018<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2017#tppubs\" >2017<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2016#tppubs\" >2016<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2015#tppubs\" >2015<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2014#tppubs\" >2014<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2013#tppubs\" >2013<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2012#tppubs\" >2012<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=2004#tppubs\" >2004<\/option><option value = \"tgid=&amp;type=&amp;auth=&amp;usr=&amp;yr=0000#tppubs\" >0000<\/option>\r\n                <\/select><select class=\"default\" name=\"type\" id=\"type\" tabindex=\"3\" onchange=\"teachpress_jumpMenu('parent',this, 'https:\/\/marte.i3a.es\/es\/micromuestreo-y-alta-resolucion-temporal-y-espacial-para-el-analisis-clinico-minimamente-invasivo-y-el-analisis-de-celulas-individuales\/?')\">\r\n                   <option value=\"tgid=&amp;yr=&amp;auth=&amp;usr=&amp;type=#tppubs\">Todas las tipolog\u00edas<\/option>\r\n                   <option value = \"tgid=&amp;yr=&amp;auth=&amp;usr=&amp;type=article#tppubs\" >Art\u00edculos de revista<\/option><option value = \"tgid=&amp;yr=&amp;auth=&amp;usr=&amp;type=proceedings#tppubs\" >Actas de congresos<\/option>\r\n                <\/select><\/div><input type=\"hidden\" name=\"trp-form-language\" value=\"es\"\/><\/form><div class=\"teachpress_publication_list\"><h3 class=\"tp_h3\" id=\"tp_h3_2025\">2025<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Rua-Ibarz, Ana;  Nakadi, Fl\u00e1vio V.;  Bolea-Fernandez, Eduardo;  Bazo, Antonio;  Battistella, Beatrice;  Matiushkina, Anna;  Resch-Genger, Ute;  Abad, Carlos;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('262','tp_links')\" style=\"cursor:pointer;\">Discrete Entity Analysis via Microwave-Induced Nitrogen Plasma\u2013Mass Spectrometry in Single-Event Mode<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 0, <\/span><span class=\"tp_pub_additional_number\">no 0, <\/span><span class=\"tp_pub_additional_pages\">pp. null, <\/span><span class=\"tp_pub_additional_year\">2025<\/span><span class=\"tp_pub_additional_note\">, (PMID: 41084806)<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('262','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('262','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('262','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_262\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{,<br \/>\r\ntitle = {Discrete Entity Analysis via Microwave-Induced Nitrogen Plasma\u2013Mass Spectrometry in Single-Event Mode},<br \/>\r\nauthor = {Ana Rua-Ibarz and Fl\u00e1vio V. Nakadi and Eduardo Bolea-Fernandez and Antonio Bazo and Beatrice Battistella and Anna Matiushkina and Ute Resch-Genger and Carlos Abad and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/doi.org\/10.1021\/acs.analchem.5c04341},<br \/>\r\ndoi = {10.1021\/acs.analchem.5c04341},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-10-14},<br \/>\r\nurldate = {2025-10-14},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {0},<br \/>\r\nnumber = {0},<br \/>\r\npages = {null},<br \/>\r\nabstract = {In this work, single-event microwave-induced nitrogen plasma\u2013mass spectrometry (single-event MINP-MS) was evaluated for the first time for the analysis of discrete entities such as nanoparticles, biological cells, and microplastics. Nitrogen (N2) effectively overcomes Ar-based polyatomic interferences, enabling (ultra)trace element determination of Fe and Se using their most abundant isotopes, 56Fe (91.66%) and 80Se (49.82%). Iron oxide nanoparticles (Fe2O3 NPs) ranging from 20 to 70 nm were accurately characterized, with excellent agreement with established sizing techniques, such as transmission electron microscopy (TEM) and dynamic light scattering (DLS). A limit of detection (LoD) of 8.6 ag for Fe\u2500equivalent to an LoDsize of 19 nm for Fe2O3\u2500was achieved, which is significantly lower than recent values reported for high-end quadrupole-based ICP-MS. Selenium nanoparticles (SeNPs) of 150 and 250 nm were also accurately characterized, without the N2-based plasma experiencing issues handling relatively large metallic NPs (linearity, R2 = 0.9994). Se-enriched yeast cells (SELM-1 certified reference material) were successfully analyzed via single-cell MINP-MS using external calibration based on SeNPs and a transport efficiency-independent approach. In addition, 2\u20133 \u03bcm polystyrene (PS) and polytetrafluoroethylene (PTFE) were accurately sized by monitoring 12C+, confirming the method\u2019s suitability for handling micrometer-sized polymeric materials (microplastics). The average duration of individual events (680 \u00b1 160 \u03bcs) suggests that the digestion of individual entities in N2-based plasmas is comparable to that in Ar-based plasmas. These results open new avenues for this instrumentation as an alternative to ICP ionization sources, also in the context of discrete entity analysis.},<br \/>\r\nnote = {PMID: 41084806},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('262','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_262\" style=\"display:none;\"><div class=\"tp_abstract_entry\">In this work, single-event microwave-induced nitrogen plasma\u2013mass spectrometry (single-event MINP-MS) was evaluated for the first time for the analysis of discrete entities such as nanoparticles, biological cells, and microplastics. Nitrogen (N2) effectively overcomes Ar-based polyatomic interferences, enabling (ultra)trace element determination of Fe and Se using their most abundant isotopes, 56Fe (91.66%) and 80Se (49.82%). Iron oxide nanoparticles (Fe2O3 NPs) ranging from 20 to 70 nm were accurately characterized, with excellent agreement with established sizing techniques, such as transmission electron microscopy (TEM) and dynamic light scattering (DLS). A limit of detection (LoD) of 8.6 ag for Fe\u2500equivalent to an LoDsize of 19 nm for Fe2O3\u2500was achieved, which is significantly lower than recent values reported for high-end quadrupole-based ICP-MS. Selenium nanoparticles (SeNPs) of 150 and 250 nm were also accurately characterized, without the N2-based plasma experiencing issues handling relatively large metallic NPs (linearity, R2 = 0.9994). Se-enriched yeast cells (SELM-1 certified reference material) were successfully analyzed via single-cell MINP-MS using external calibration based on SeNPs and a transport efficiency-independent approach. In addition, 2\u20133 \u03bcm polystyrene (PS) and polytetrafluoroethylene (PTFE) were accurately sized by monitoring 12C+, confirming the method\u2019s suitability for handling micrometer-sized polymeric materials (microplastics). The average duration of individual events (680 \u00b1 160 \u03bcs) suggests that the digestion of individual entities in N2-based plasmas is comparable to that in Ar-based plasmas. These results open new avenues for this instrumentation as an alternative to ICP ionization sources, also in the context of discrete entity analysis.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('262','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_262\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/doi.org\/10.1021\/acs.analchem.5c04341\" title=\"https:\/\/doi.org\/10.1021\/acs.analchem.5c04341\" target=\"_blank\">https:\/\/doi.org\/10.1021\/acs.analchem.5c04341<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.1021\/acs.analchem.5c04341\" title=\"DOI de seguimiento:10.1021\/acs.analchem.5c04341\" target=\"_blank\">doi:10.1021\/acs.analchem.5c04341<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('262','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Bazo, Antonio;  Bolea-Fernandez, Eduardo;  Rua-Ibarz, Ana;  Aramend\u00eda, Maite;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('258','tp_links')\" style=\"cursor:pointer;\">Ions with Ions, Entities with Entities: A Proof-of-Concept Study Using the SELM-1 Yeast Certified Reference Material for Intra- and Extracellular Se Quantification via Single-Cell ICP-Mass Spectrometry<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Anal. Chem., <\/span><span class=\"tp_pub_additional_year\">2025<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 1520-6882<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('258','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('258','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('258','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_258\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Ions with Ions, Entities with Entities: A Proof-of-Concept Study Using the SELM-1 Yeast Certified Reference Material for Intra- and Extracellular Se Quantification via Single-Cell ICP-Mass Spectrometry},<br \/>\r\nauthor = {Antonio Bazo and Eduardo Bolea-Fernandez and Ana Rua-Ibarz and Maite Aramend\u00eda and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c01588},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1021\/acs.analchem.5c01588},<br \/>\r\nissn = {1520-6882},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-06-07},<br \/>\r\nurldate = {2025-06-07},<br \/>\r\njournal = {Anal. Chem.},<br \/>\r\nabstract = {In this work, two novel nanoparticle (NP)-based calibration strategies, external calibration and a relative method, have been explored for single-cell ICP-mass spectrometry (SC-ICP-MS) analysis. The fundamental principle of these methods is to rely on individual entities (well-characterized NPs of the target analyte) for calibration rather than on ionic standard solutions. The performance of the NP-based calibration approaches has been compared to that of the reference method (particle size with AuNP standards). In addition to the intracellular Se content (mass per individual cell), the extracellular Se (dissolved fraction) was also determined directly and simultaneously using the average background from the SC-ICP-MS time-resolved signal. The figures-of-merit of the methods developed have been evaluated by relying on the analysis of the SELM-1 cell-certified reference material, consisting of Se-enriched yeast cells, and certified for its total Se content (intracellular + extracellular Se). All methods successfully determined the Se elemental contents, but an improvement in accuracy and precision was observed for the NP-based methods compared to the reference one. Furthermore, the NP-based methods were found to be less time-consuming, more straightforward, and more user-friendly in terms of calculations. These results open new avenues for calibration in quantitative SC-ICP-MS analysis and call for a fundamental change in the methodology, where the determination of ionic contents is based on the use of ionic standard solutions for calibration, while the determination of elemental contents in discrete micro\/nanoentities, such as cells, should ideally be based on calibration using standard entities, thus avoiding the need to calculate a transport efficiency coefficient.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('258','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_258\" style=\"display:none;\"><div class=\"tp_abstract_entry\">In this work, two novel nanoparticle (NP)-based calibration strategies, external calibration and a relative method, have been explored for single-cell ICP-mass spectrometry (SC-ICP-MS) analysis. The fundamental principle of these methods is to rely on individual entities (well-characterized NPs of the target analyte) for calibration rather than on ionic standard solutions. The performance of the NP-based calibration approaches has been compared to that of the reference method (particle size with AuNP standards). In addition to the intracellular Se content (mass per individual cell), the extracellular Se (dissolved fraction) was also determined directly and simultaneously using the average background from the SC-ICP-MS time-resolved signal. The figures-of-merit of the methods developed have been evaluated by relying on the analysis of the SELM-1 cell-certified reference material, consisting of Se-enriched yeast cells, and certified for its total Se content (intracellular + extracellular Se). All methods successfully determined the Se elemental contents, but an improvement in accuracy and precision was observed for the NP-based methods compared to the reference one. Furthermore, the NP-based methods were found to be less time-consuming, more straightforward, and more user-friendly in terms of calculations. These results open new avenues for calibration in quantitative SC-ICP-MS analysis and call for a fundamental change in the methodology, where the determination of ionic contents is based on the use of ionic standard solutions for calibration, while the determination of elemental contents in discrete micro\/nanoentities, such as cells, should ideally be based on calibration using standard entities, thus avoiding the need to calculate a transport efficiency coefficient.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('258','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_258\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c01588\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c01588\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.5c01588<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1021\/acs.analchem.5c01588\" title=\"DOI de seguimiento:https:\/\/doi.org\/10.1021\/acs.analchem.5c01588\" target=\"_blank\">doi:https:\/\/doi.org\/10.1021\/acs.analchem.5c01588<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('258','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Nakadi, Fl\u00e1vio V.;  Garcia-Garcia, Alicia;  Rua-Ibarz, Ana;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('254','tp_links')\" style=\"cursor:pointer;\">LAMIS in the gas phase: A new approach for obtaining Ca elemental and isotopic information via CaF molecule formation<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Talanta, <\/span><span class=\"tp_pub_additional_volume\">vol. 292, <\/span><span class=\"tp_pub_additional_pages\">pp. 127920, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 0039-9140<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('254','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('254','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('254','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_254\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{NAKADI2025127920,<br \/>\r\ntitle = {LAMIS in the gas phase: A new approach for obtaining Ca elemental and isotopic information via CaF molecule formation},<br \/>\r\nauthor = {Fl\u00e1vio V. Nakadi and Alicia Garcia-Garcia and Ana Rua-Ibarz and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0039914025004102},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.talanta.2025.127920},<br \/>\r\nissn = {0039-9140},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-01-01},<br \/>\r\nurldate = {2025-01-01},<br \/>\r\njournal = {Talanta},<br \/>\r\nvolume = {292},<br \/>\r\npages = {127920},<br \/>\r\nabstract = {This work introduces a novel method for generating the calcium monofluoride (CaF) diatomic molecule by adding the molecule-forming reagent in the gaseous phase (a methyl fluoride-argon mixture), in order to perform laser-induced breakdown spectroscopy (LIBS) and laser ablation molecular isotopic spectrometry (LAMIS) measurements. By optimizing the instrumental parameters, CaF molecule formation was successfully achieved within the plasma plume, upon ablation of dried liquid samples. The isotopic shift for the X2\u03a3\u2192A2\u03a0 (0,1) CaF vibronic transition at 583.0\u00a0nm was calculated to be 292.3 pm. The method proved capable of providing quantitative information for determining calcium concentrations in real samples, such as tap water and skimmed milk, using internal standardization (with Sr as internal standard; limit of detection, LOD, 20\u00a0mg\u00a0L\u22121) and isotope dilution (which can be applied from 400\u00a0mg\u00a0L\u22121on), respectively. Partial least squares regression (PLS) analysis was employed to enhance the quality of the isotopic data. The Ca concentration found in the tap water was 47\u00a0\u00b1\u00a016\u00a0mg\u00a0L\u22121 (reference flame atomic absorption spectrometry, FAAS, value: 59\u00a0\u00b1\u00a00.2\u00a0mg\u00a0L\u22121), and 1100\u00a0\u00b1\u00a0140\u00a0mg\u00a0L\u22121 for the skimmed milk (reference FAAS value: 1240\u00a0\u00b1\u00a0120\u00a0mg\u00a0L\u22121). No significant difference between LIBS and FAAS results could be established using a t-test at the 95% confidence level. Overall, this novel approach allows for the determination of calcium in terms of both the elemental concentration and the isotopic composition, thus broadening the applicability of LIBS (e.g., for tracer experiments, besides the already mentioned application of isotope dilution).},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('254','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_254\" style=\"display:none;\"><div class=\"tp_abstract_entry\">This work introduces a novel method for generating the calcium monofluoride (CaF) diatomic molecule by adding the molecule-forming reagent in the gaseous phase (a methyl fluoride-argon mixture), in order to perform laser-induced breakdown spectroscopy (LIBS) and laser ablation molecular isotopic spectrometry (LAMIS) measurements. By optimizing the instrumental parameters, CaF molecule formation was successfully achieved within the plasma plume, upon ablation of dried liquid samples. The isotopic shift for the X2\u03a3\u2192A2\u03a0 (0,1) CaF vibronic transition at 583.0\u00a0nm was calculated to be 292.3 pm. The method proved capable of providing quantitative information for determining calcium concentrations in real samples, such as tap water and skimmed milk, using internal standardization (with Sr as internal standard; limit of detection, LOD, 20\u00a0mg\u00a0L\u22121) and isotope dilution (which can be applied from 400\u00a0mg\u00a0L\u22121on), respectively. Partial least squares regression (PLS) analysis was employed to enhance the quality of the isotopic data. The Ca concentration found in the tap water was 47\u00a0\u00b1\u00a016\u00a0mg\u00a0L\u22121 (reference flame atomic absorption spectrometry, FAAS, value: 59\u00a0\u00b1\u00a00.2\u00a0mg\u00a0L\u22121), and 1100\u00a0\u00b1\u00a0140\u00a0mg\u00a0L\u22121 for the skimmed milk (reference FAAS value: 1240\u00a0\u00b1\u00a0120\u00a0mg\u00a0L\u22121). No significant difference between LIBS and FAAS results could be established using a t-test at the 95% confidence level. Overall, this novel approach allows for the determination of calcium in terms of both the elemental concentration and the isotopic composition, thus broadening the applicability of LIBS (e.g., for tracer experiments, besides the already mentioned application of isotope dilution).<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('254','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_254\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0039914025004102\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0039914025004102\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0039914025004102<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.talanta.2025.127920\" title=\"DOI de seguimiento:https:\/\/doi.org\/10.1016\/j.talanta.2025.127920\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.talanta.2025.127920<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('254','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Bazo, Antonio;  Bolea-Fernandez, Eduardo;  Billimoria, Kharmen;  Rua-Ibarz, Ana;  Aramend\u00eda, Maite;  Menero-Vald\u00e9s, Paula;  Morley, Jack;  Neves, Sara;  S\u00e1nchez-Cachero, Armando;  Goenaga-Infante, Heidi;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('259','tp_links')\" style=\"cursor:pointer;\">A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">J. Anal. At. Spectrom., <\/span><span class=\"tp_pub_additional_pages\">pp. -, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_259\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('259','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_259\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('259','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_259\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('259','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_259\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{D5JA00253B,<br \/>\r\ntitle = {A novel particle mass calibration strategy for the quantification of AuNPs in single cancer cells via laser ablation ICP-mass spectrometry. A case study},<br \/>\r\nauthor = {Antonio Bazo and Eduardo Bolea-Fernandez and Kharmen Billimoria and Ana Rua-Ibarz and Maite Aramend\u00eda and Paula Menero-Vald\u00e9s and Jack Morley and Sara Neves and Armando S\u00e1nchez-Cachero and Heidi Goenaga-Infante and Mart\u00edn Resano},<br \/>\r\nurl = {http:\/\/dx.doi.org\/10.1039\/D5JA00253B},<br \/>\r\ndoi = {10.1039\/D5JA00253B},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-01-01},<br \/>\r\nurldate = {2025-01-01},<br \/>\r\njournal = {J. Anal. At. Spectrom.},<br \/>\r\npages = {-},<br \/>\r\npublisher = {The Royal Society of Chemistry},<br \/>\r\nabstract = {Laser ablation ICP-mass spectrometry (LA-ICP-MS) has developed as a powerful tool for elemental quantitative analysis of individual cells, assuring that the content of each cell is analyzed individually. However, this technique is still limited by the difficulties associated with calibration using solid standards. This work proposes a particle mass calibration strategy that is independent of both the properties and thickness of the gelatin films used for calibration, overcoming a significant drawback of previously established methods. The fundamental principle of this strategy relies on the individual ablation of nanoparticles (NPs) of well-characterized size that are embedded in the films, so that their mass can be directly used for calibration without the need to calculate their exact concentration within the gelatin. The performance of the newly developed method was compared to that of the previously reported approaches (ionic and particle number calibration) in terms of linearity and homogeneity between different films prepared from the same gelatin solution. As a case study, the three calibration strategies were used for the quantitative analysis of HeLa cancer cells exposed to AuNPs. In parallel, in-suspension single-cell (SC) ICP-MS Au data were obtained and used as reference for comparison with the three LA-SC-ICP-MS strategies. The results obtained with the novel particle mass approach demonstrated better accuracy and repeatability over three different working sessions, addressing key limitations and providing a robust and reliable method for quantitative LA-SC-ICP-MS analysis. The particle mass method holds promise for quantitative LA-ICP-MS analysis of samples beyond NP-exposed cells, such as biological tissues.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('259','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_259\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Laser ablation ICP-mass spectrometry (LA-ICP-MS) has developed as a powerful tool for elemental quantitative analysis of individual cells, assuring that the content of each cell is analyzed individually. However, this technique is still limited by the difficulties associated with calibration using solid standards. This work proposes a particle mass calibration strategy that is independent of both the properties and thickness of the gelatin films used for calibration, overcoming a significant drawback of previously established methods. The fundamental principle of this strategy relies on the individual ablation of nanoparticles (NPs) of well-characterized size that are embedded in the films, so that their mass can be directly used for calibration without the need to calculate their exact concentration within the gelatin. The performance of the newly developed method was compared to that of the previously reported approaches (ionic and particle number calibration) in terms of linearity and homogeneity between different films prepared from the same gelatin solution. As a case study, the three calibration strategies were used for the quantitative analysis of HeLa cancer cells exposed to AuNPs. In parallel, in-suspension single-cell (SC) ICP-MS Au data were obtained and used as reference for comparison with the three LA-SC-ICP-MS strategies. The results obtained with the novel particle mass approach demonstrated better accuracy and repeatability over three different working sessions, addressing key limitations and providing a robust and reliable method for quantitative LA-SC-ICP-MS analysis. The particle mass method holds promise for quantitative LA-ICP-MS analysis of samples beyond NP-exposed cells, such as biological tissues.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('259','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_259\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"http:\/\/dx.doi.org\/10.1039\/D5JA00253B\" title=\"http:\/\/dx.doi.org\/10.1039\/D5JA00253B\" target=\"_blank\">http:\/\/dx.doi.org\/10.1039\/D5JA00253B<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.1039\/D5JA00253B\" title=\"DOI de seguimiento:10.1039\/D5JA00253B\" target=\"_blank\">doi:10.1039\/D5JA00253B<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('259','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Bazo, Antonio;  L\u00f3pez-Villellas, Lori\u00e9n;  Mataloni, Matilde;  Bolea-Fernandez, Eduardo;  Rua-Ibarz, Ana;  Grotti, Marco;  Aramend\u00eda, Maite;  Resano, Mart\u00edn<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('260','tp_links')\" style=\"cursor:pointer;\">Improving detection and figures of merit in single-particle inductively coupled plasma-mass spectrometry via transient event heights<\/a> <span class=\"tp_pub_type tp_  article\">Art\u00edculo de revista<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">En: <\/span><span class=\"tp_pub_additional_journal\">Analytica Chimica Acta, <\/span><span class=\"tp_pub_additional_volume\">vol. 1378, <\/span><span class=\"tp_pub_additional_pages\">pp. 344694, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 0003-2670<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('260','tp_abstract')\" title=\"Mostrar resumen\" style=\"cursor:pointer;\">Resumen<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('260','tp_links')\" title=\"Mostrar enlaces y recursos\" style=\"cursor:pointer;\">Enlaces<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('260','tp_bibtex')\" title=\"Mostrar entrada BibTeX \" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_260\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{BAZO2025344694,<br \/>\r\ntitle = {Improving detection and figures of merit in single-particle inductively coupled plasma-mass spectrometry via transient event heights},<br \/>\r\nauthor = {Antonio Bazo and Lori\u00e9n L\u00f3pez-Villellas and Matilde Mataloni and Eduardo Bolea-Fernandez and Ana Rua-Ibarz and Marco Grotti and Maite Aramend\u00eda and Mart\u00edn Resano},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267025010888},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.aca.2025.344694},<br \/>\r\nissn = {0003-2670},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-01-01},<br \/>\r\nurldate = {2025-01-01},<br \/>\r\njournal = {Analytica Chimica Acta},<br \/>\r\nvolume = {1378},<br \/>\r\npages = {344694},<br \/>\r\nabstract = {Background <br \/>\r\nSingle-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) is a powerful method for characterizing micro- and nanoparticulate materials. The technique primarily relies on the linear relationship between the integrated intensities of individual events (peak areas) and the analyte mass, though transit times (peak widths) have also been used for quantitative purposes. This work (1) evaluates the potential of using peak heights as analytical signals in SP-ICP-MS, (2) introduces a new method for determining peak heights, and (3) explores scenarios in which peak height offers added value over the commonly used SP-ICP-MS signals. <br \/>\r\nResults <br \/>\r\nA new method was proposed to estimate peak height values in SP-ICP-MS accurately. The cumulative intensity across consecutive dwell times was modeled using a third-degree polynomial, from which the adjusted peak height was derived. This approach reduces the uncertainty associated with using raw maximum intensity values, yielding NP distributions comparable to those obtained via integrated intensities. The effect of dwell time on peak height was also evaluated. An optimal range (50 \u03bcs\u2013200 \u03bcs) was identified, where a linear relationship was observed between the peak height and the square of the NP diameter. Within this range, peak height showed the lowest bias when characterizing smaller NPs, indicating the potential to improve the limit of quantification (LoQ). Additionally, peak heights proved helpful in determining the limit of detection (LoD) and setting appropriate threshold values for data processing, thereby helping to flag incorrect resultsand addressing a challenge in SP-ICP-MS analysis. <br \/>\r\nSignificance <br \/>\r\nThis is the first study to evaluate peak height as an analytical signal in SP-ICP-MS. The results highlight its advantages in specific applications, such as sizing NPs near the LoD, and in supporting the more reliable use of other signals, such as peak areas, by helping to identify incorrect threshold selection that could lead to biased distributions. Finally, monitoring peak heights allows for a more realistic and assumption-free determination of the LoD.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('260','tp_bibtex')\">Cerrar<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_260\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Background <br \/>\r\nSingle-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) is a powerful method for characterizing micro- and nanoparticulate materials. The technique primarily relies on the linear relationship between the integrated intensities of individual events (peak areas) and the analyte mass, though transit times (peak widths) have also been used for quantitative purposes. This work (1) evaluates the potential of using peak heights as analytical signals in SP-ICP-MS, (2) introduces a new method for determining peak heights, and (3) explores scenarios in which peak height offers added value over the commonly used SP-ICP-MS signals. <br \/>\r\nResults <br \/>\r\nA new method was proposed to estimate peak height values in SP-ICP-MS accurately. The cumulative intensity across consecutive dwell times was modeled using a third-degree polynomial, from which the adjusted peak height was derived. This approach reduces the uncertainty associated with using raw maximum intensity values, yielding NP distributions comparable to those obtained via integrated intensities. The effect of dwell time on peak height was also evaluated. An optimal range (50 \u03bcs\u2013200 \u03bcs) was identified, where a linear relationship was observed between the peak height and the square of the NP diameter. Within this range, peak height showed the lowest bias when characterizing smaller NPs, indicating the potential to improve the limit of quantification (LoQ). Additionally, peak heights proved helpful in determining the limit of detection (LoD) and setting appropriate threshold values for data processing, thereby helping to flag incorrect resultsand addressing a challenge in SP-ICP-MS analysis. <br \/>\r\nSignificance <br \/>\r\nThis is the first study to evaluate peak height as an analytical signal in SP-ICP-MS. The results highlight its advantages in specific applications, such as sizing NPs near the LoD, and in supporting the more reliable use of other signals, such as peak areas, by helping to identify incorrect threshold selection that could lead to biased distributions. Finally, monitoring peak heights allows for a more realistic and assumption-free determination of the LoD.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('260','tp_abstract')\">Cerrar<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_260\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267025010888\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267025010888\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267025010888<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.aca.2025.344694\" title=\"DOI de seguimiento:https:\/\/doi.org\/10.1016\/j.aca.2025.344694\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.aca.2025.344694<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('260','tp_links')\">Cerrar<\/a><\/p><\/div><\/div><\/div><\/div><\/div>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":3,"featured_media":4282,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[226,230],"tags":[],"class_list":["post-4278","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-featured-projects","category-projects"],"_links":{"self":[{"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/posts\/4278","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/comments?post=4278"}],"version-history":[{"count":13,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/posts\/4278\/revisions"}],"predecessor-version":[{"id":4407,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/posts\/4278\/revisions\/4407"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/media\/4282"}],"wp:attachment":[{"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/media?parent=4278"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/categories?post=4278"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/marte.i3a.es\/es\/wp-json\/wp\/v2\/tags?post=4278"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}