Call: +34 976 765 500 ext 142156
Email: lrello@salud.aragon.es
Address: c/Padre Arrupe sn, Hospital Universitario Miguel Servet, S° Bioquímica Clínica – Zaragoza (Spain)
ABOUT ME
PUBLICATIONS
2013
Aramendía, Maite; Rello, Luis; Resano, Martín; Vanhaecke, Frank
Isotopic analysis of Cu in serum samples for diagnosis of Wilson’s disease: a pilot study Journal Article
En: J. Anal. At. Spectrom., vol. 28, iss. 5, pp. 675-681, 2013.
@article{C3JA30349G,
title = {Isotopic analysis of Cu in serum samples for diagnosis of Wilson's disease: a pilot study},
author = {Maite Aramendía and Luis Rello and Martín Resano and Frank Vanhaecke},
url = {http://dx.doi.org/10.1039/C3JA30349G},
doi = {10.1039/C3JA30349G},
year = {2013},
date = {2013-01-01},
journal = {J. Anal. At. Spectrom.},
volume = {28},
issue = {5},
pages = {675-681},
publisher = {The Royal Society of Chemistry},
abstract = {Wilson's disease (WD) is a genetic disorder affecting Cu metabolism, which can lead to severe physiological and neurological symptoms, and even death if untreated. Based on the fact that WD patients show low Cu levels in serum, implementation of screening programs for diagnosis of this condition at the moment of birth, when progression of the disease can be still arrested, has been attempted in the past. These attempts, however, have been unsuccessful, as healthy new-borns often show low Cu levels in serum due to liver immaturity. In this work, the potential use of isotopic analysis of Cu in serum samples as an alternative diagnostic parameter for Wilson's disease has been investigated. For this purpose, the Cu isotopic composition of a set of serum samples from different groups showing either low (i.e. WD patients, patients who had undergone bariatric surgery, infants) or normal (supposedly healthy adults) Cu concentration levels was determined by means of multi-collector ICP-mass spectrometry (MC-ICP-MS), after chromatographic isolation of Cu. For this purpose, AG-MP-1 strong anion exchange resin was relied upon, enabling quantitative recovery of Cu in pure form from the serum samples. MC-ICP-MS measuring conditions were optimized to avoid the influence of spectral overlap, and Ni was admixed as an internal standard for correction of instrumental mass discrimination. The use of this optimized method provided δ65Cu for the serum samples with a typical analytical uncertainty of ±0.20‰ (k = 2). Our results show that, for the population considered in this study, combination of Cu concentration values and Cu isotopic information allows classification of WD patients, infants and controls into different groups, while the use of concentration values only is not sufficient for this purpose. Although further studies with a larger number of samples are needed, results are encouraging as far as the use of Cu isotopic analysis for early diagnosis of Wilson's disease is concerned.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Wilson’s disease (WD) is a genetic disorder affecting Cu metabolism, which can lead to severe physiological and neurological symptoms, and even death if untreated. Based on the fact that WD patients show low Cu levels in serum, implementation of screening programs for diagnosis of this condition at the moment of birth, when progression of the disease can be still arrested, has been attempted in the past. These attempts, however, have been unsuccessful, as healthy new-borns often show low Cu levels in serum due to liver immaturity. In this work, the potential use of isotopic analysis of Cu in serum samples as an alternative diagnostic parameter for Wilson’s disease has been investigated. For this purpose, the Cu isotopic composition of a set of serum samples from different groups showing either low (i.e. WD patients, patients who had undergone bariatric surgery, infants) or normal (supposedly healthy adults) Cu concentration levels was determined by means of multi-collector ICP-mass spectrometry (MC-ICP-MS), after chromatographic isolation of Cu. For this purpose, AG-MP-1 strong anion exchange resin was relied upon, enabling quantitative recovery of Cu in pure form from the serum samples. MC-ICP-MS measuring conditions were optimized to avoid the influence of spectral overlap, and Ni was admixed as an internal standard for correction of instrumental mass discrimination. The use of this optimized method provided δ65Cu for the serum samples with a typical analytical uncertainty of ±0.20‰ (k = 2). Our results show that, for the population considered in this study, combination of Cu concentration values and Cu isotopic information allows classification of WD patients, infants and controls into different groups, while the use of concentration values only is not sufficient for this purpose. Although further studies with a larger number of samples are needed, results are encouraging as far as the use of Cu isotopic analysis for early diagnosis of Wilson’s disease is concerned.
Rello, L.; Lapeña, A. C.; Aramendía, M.; Belarra, M. A.; Resano, M.
En: Spectrochimica Acta Part B: Atomic Spectroscopy, vol. 81, pp. 11-19, 2013, ISSN: 0584-8547.
@article{RELLO201311,
title = {A dried urine spot test to simultaneously monitor Mo and Ti levels using solid sampling high-resolution continuum source graphite furnace atomic absorption spectrometry},
author = {L. Rello and A. C. Lapeña and M. Aramendía and M. A. Belarra and M. Resano},
url = {https://www.sciencedirect.com/science/article/pii/S0584854712003734},
doi = {https://doi.org/10.1016/j.sab.2012.12.001},
issn = {0584-8547},
year = {2013},
date = {2013-01-01},
journal = {Spectrochimica Acta Part B: Atomic Spectroscopy},
volume = {81},
pages = {11-19},
abstract = {Home-based collection protocols for clinical specimens are actively pursued as a means of improving life quality of patients that require frequent controls, such as patients with metallic prosthesis, for whom monitoring the evolution of Mo and Ti in biological fluids may play a decisive role to detect prosthesis mal-functioning. The collection of biological fluids on clinical filter papers provides a simple way to implement these protocols. This work explores the potential of solid sampling high-resolution continuum source graphite furnace atomic absorption spectrometry for the simultaneous and direct determination of Mo and Ti in urine, after its deposition onto clinical filter paper, giving rise to a dried urine spot. The approach used for depositing the sample was found crucial to develop a quantitative method, since the filter paper acts as a chromatographic support and produces a differential distribution of the target analytes. Furthermore, the high spreading of urine onto a filter paper results in a small amount of urine per surface unit, and thus, ultimately, in lack of sensitivity. In order to circumvent these problems, the use of an alternative approach based on the use of pre-cut 17×19mm filter paper pieces onto which larger amounts of sample (500μL) can be retained by single deposition was proposed and evaluated. In this way, an approximately 12-fold increase in sensitivity and a more homogeneous distribution of the target analytes were obtained, permitting the development of a quantification strategy based on the use of matrix-matched urine samples of known analyte concentrations, which were subjected to the same procedure as the samples. Accuracy of this method, which provides LODs of 1.5μgL−1 for Mo and 6.5μgL−1 for Ti, was demonstrated after analysis of urine reference materials. Overall, the performance of the method developed is promising, being likely suitable for determination of other analytes in dried urine spots.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Home-based collection protocols for clinical specimens are actively pursued as a means of improving life quality of patients that require frequent controls, such as patients with metallic prosthesis, for whom monitoring the evolution of Mo and Ti in biological fluids may play a decisive role to detect prosthesis mal-functioning. The collection of biological fluids on clinical filter papers provides a simple way to implement these protocols. This work explores the potential of solid sampling high-resolution continuum source graphite furnace atomic absorption spectrometry for the simultaneous and direct determination of Mo and Ti in urine, after its deposition onto clinical filter paper, giving rise to a dried urine spot. The approach used for depositing the sample was found crucial to develop a quantitative method, since the filter paper acts as a chromatographic support and produces a differential distribution of the target analytes. Furthermore, the high spreading of urine onto a filter paper results in a small amount of urine per surface unit, and thus, ultimately, in lack of sensitivity. In order to circumvent these problems, the use of an alternative approach based on the use of pre-cut 17×19mm filter paper pieces onto which larger amounts of sample (500μL) can be retained by single deposition was proposed and evaluated. In this way, an approximately 12-fold increase in sensitivity and a more homogeneous distribution of the target analytes were obtained, permitting the development of a quantification strategy based on the use of matrix-matched urine samples of known analyte concentrations, which were subjected to the same procedure as the samples. Accuracy of this method, which provides LODs of 1.5μgL−1 for Mo and 6.5μgL−1 for Ti, was demonstrated after analysis of urine reference materials. Overall, the performance of the method developed is promising, being likely suitable for determination of other analytes in dried urine spots.
2012
Aramendía, Maite; Rello, Luis; Vanhaecke, Frank; Resano, Martín
En: Anal Chem, vol. 84, no. 20, pp. 8682–8690, 2012, ISSN: 1520-6882.
@article{pmid22935036,
title = {Direct trace-elemental analysis of urine samples by laser ablation-inductively coupled plasma mass spectrometry after sample deposition on clinical filter papers},
author = {Maite Aramendía and Luis Rello and Frank Vanhaecke and Martín Resano},
doi = {10.1021/ac3018839},
issn = {1520-6882},
year = {2012},
date = {2012-10-01},
journal = {Anal Chem},
volume = {84},
number = {20},
pages = {8682--8690},
abstract = {Collection of biological fluids on clinical filter papers shows important advantages from a logistic point of view, although analysis of these specimens is far from straightforward. Concerning urine analysis, and particularly when direct trace elemental analysis by laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) is aimed at, several problems arise, such as lack of sensitivity or different distribution of the analytes on the filter paper, rendering obtaining reliable quantitative results quite difficult. In this paper, a novel approach for urine collection is proposed, which circumvents many of these problems. This methodology consists on the use of precut filter paper discs where large amounts of sample can be retained upon a single deposition. This provides higher amounts of the target analytes and, thus, sufficient sensitivity, and allows addition of an adequate internal standard at the clinical lab prior to analysis, therefore making it suitable for a strategy based on unsupervised sample collection and ulterior analysis at referral centers. On the basis of this sampling methodology, an analytical method was developed for the direct determination of several elements in urine (Be, Bi, Cd, Co, Cu, Ni, Sb, Sn, Tl, Pb, and V) at the low μg L(-1) level by means of LA-ICPMS. The method developed provides good results in terms of accuracy and LODs (≤1 μg L(-1) for most of the analytes tested), with a precision in the range of 15%, fit-for-purpose for clinical control analysis.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Collection of biological fluids on clinical filter papers shows important advantages from a logistic point of view, although analysis of these specimens is far from straightforward. Concerning urine analysis, and particularly when direct trace elemental analysis by laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) is aimed at, several problems arise, such as lack of sensitivity or different distribution of the analytes on the filter paper, rendering obtaining reliable quantitative results quite difficult. In this paper, a novel approach for urine collection is proposed, which circumvents many of these problems. This methodology consists on the use of precut filter paper discs where large amounts of sample can be retained upon a single deposition. This provides higher amounts of the target analytes and, thus, sufficient sensitivity, and allows addition of an adequate internal standard at the clinical lab prior to analysis, therefore making it suitable for a strategy based on unsupervised sample collection and ulterior analysis at referral centers. On the basis of this sampling methodology, an analytical method was developed for the direct determination of several elements in urine (Be, Bi, Cd, Co, Cu, Ni, Sb, Sn, Tl, Pb, and V) at the low μg L(-1) level by means of LA-ICPMS. The method developed provides good results in terms of accuracy and LODs (≤1 μg L(-1) for most of the analytes tested), with a precision in the range of 15%, fit-for-purpose for clinical control analysis.