Publications

@article{GARCIAPOYO2021106306,

title = {Evaluation of electrothermal vaporization for sample introduction aiming at Cu isotopic analysis via multicollector-inductively coupled plasma mass spectrometry},

author = {M. Carmen García-Poyo and Anne Laure Ronzani and Jérôme Frayret and Sylvain Bérail and Luis Rello and Elena García-González and Bénédicte Lelièvre and Flávio V. Nakadi and Maite Aramendía and Martín Resano and Christophe Pécheyran},

url = {https://www.sciencedirect.com/science/article/pii/S0584854721002639},

doi = {https://doi.org/10.1016/j.sab.2021.106306},

issn = {0584-8547},

year  = {2021},

date = {2021-01-01},

journal = {Spectrochimica Acta Part B: Atomic Spectroscopy},

volume = {185},

pages = {106306},

abstract = {A new method for Cu isotopic analysis was developed using a commercially available electrothermal vaporization (ETV) device coupled to multicollector-inductively coupled plasma mass spectrometry (MC-ICP-MS). The method demonstrated potential for the isotopic analysis of microsamples (e.g., 5 μL) in a biological context. For example, Cu isotopic analysis of NIST 3114 (diluted to 1 mg L−1 Cu) using self-bracketing provided average δ65Cu values of 0.00 ± 0.17‰ (2SD},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{D1JA00155H,

title = {Determination of Cu in blood via direct analysis of dried blood spots using high-resolution continuum source graphite furnace atomic absorption spectrometry},

author = {M. Carmen García-Poyo and Christophe Pécheyran and Luis Rello and Elena García-González and Sharay Alonso Rodríguez and Flávio V. Nakadi and Maite Aramendía and Martín Resano},

url = {http://dx.doi.org/10.1039/D1JA00155H},

doi = {10.1039/D1JA00155H},

year  = {2021},

date = {2021-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {36},

issue = {8},

pages = {1666-1677},

publisher = {The Royal Society of Chemistry},

abstract = {The performance of state-of-the-art high-resolution continuum source graphite furnace atomic absorption spectrometry (HR CS GFAAS) instrumentation and four novel devices to produce dried blood spots of perfectly defined volumes is evaluated with the aim of developing a simple, direct method for the determination of Cu in blood samples. In all cases, it is feasible to obtain accurate quantitative information using any of the four devices tested (Mitra, HemaXis DB10, Capitainer qDBS and HemaPEN) via simple external calibration with aqueous standards. One of the main differences in the performance of such devices is related to the blanks obtained, such that HemaXis DB10 and HemaPEN are preferred when abnormally low Cu levels (500 μg L−1 or lower), associated with some diseases, need to be determined. The results prove that accurate values with RSD values below 10% can be achieved for these devices even for such Cu levels, while for Capitainer qDBS and, to a higher extent, for Mitra, blank variations will ultimately increase the uncertainty. It is important to stress that analysis of four real samples using both venipuncture and all the DBS specimens showed a very good agreement. Thus, the approach proposed could be readily applied, such that patients with disorders requiring Cu control can prepare their own samples and submit them via postal mail to labs for HR CS GFAAS direct and fast analysis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{D1JA00068C,

title = {An insight into the determination of size and number concentration of silver nanoparticles in blood using single particle ICP-MS (spICP-MS): feasibility of application to samples relevant to in vivo toxicology studies},

author = {I. Abad-Alvaro and D. Leite and D. Bartczak and S. Cuello-Nunez and B. Gomez-Gomez and Y. Madrid and M. Aramendia and M. Resano and H. Goenaga-Infante},

url = {http://dx.doi.org/10.1039/D1JA00068C},

doi = {10.1039/D1JA00068C},

year  = {2021},

date = {2021-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {36},

issue = {6},

pages = {1180-1192},

publisher = {The Royal Society of Chemistry},

abstract = {Toxicological studies concerning nanomaterials in complex biological matrices usually require a carefully designed workflow that involves handling, transportation and preparation of a large number of samples without affecting the nanoparticle (NP) characteristics, as well as measurement methods that enable their reliable characterisation. This work describes method development for the determination of number concentration and size of silver nanoparticles (AgNP) in blood for the purpose of application to the typical workflow of an in vivo toxicology assessment involving blood-containing AgNP and Ag(i) leached out from medical devices. A systematic comparison of single particle detection using millisecond versus microsecond dwell times in the presence of different ionic Ag [Ag(i)]-to-AgNP ratios by spICP-MS was undertaken to achieve sufficient selectivity for the determination of NP number concentration. This was achieved for the first time in a complex media such as 2.5% tetramethylammonium hydroxide (TMAH)/0.1% Triton X-100 (v/v) diluent, which was proven to preserve nanoparticle stability upon 8 days of storage following AgNP quantitative extraction from the blood matrix. The potential of microsecond dwell time for improved discrimination of AgNP (40 nm) from Ag(i) was demonstrated for ionic to nanoparticle ratios [Ag(i)/AgNP] of up to 106-fold. For NP sizing, a systematic study of the impact of matrix-matched ionic calibration on the derived particle size by spICP-MS is also described. Three different ionic calibration media including 1% HNO3 (v/v), 1 mM trisodium citrate and 2.5% TMAH/0.1% Triton X-100 (v/v) were tested. Student t-test evidenced statistically significant differences between the slope of the calibration curve of Ag(i) in TMAH/Triton X-100 compared to HNO3 and trisodium citrate matrices, whereas no significant differences were found between the two latter media. Moreover, a good agreement was found between the particle diameter derived from spICP-MS following ionic calibration in TMAH/Triton X-100 and the diameter obtained with transmission electron microscopy (TEM), demonstrating the importance of matrix-matched calibration for NP size determination in a complex matrix using spICP-MS. Number concentration recovery measurements on blood samples spiked with AgNP and size measurements both using spICP-MS demonstrated the feasibility of the methodology developed here for potential future application to AgNP characterisation in toxicology research.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{D0JA00494D,

title = {Laser ablation of microdroplets for copper isotopic analysis via MC-ICP-MS. Analysis of serum microsamples for the diagnosis and follow-up treatment of Wilson's disease},

author = {M. Carmen García-Poyo and Sylvain Bérail and Anne Laure Ronzani and Luis Rello and Elena García-González and Bénédicte Lelièvre and Paul Cales and Flavio V. Nakadi and Maite Aramendía and Martín Resano and Christophe Pécheyran},

url = {http://dx.doi.org/10.1039/D0JA00494D},

doi = {10.1039/D0JA00494D},

year  = {2021},

date = {2021-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {36},

issue = {5},

pages = {968-980},

publisher = {The Royal Society of Chemistry},

abstract = {Cu isotopic analysis can provide valuable insight when investigating Wilson's disease (WD), but one of the problems related to this type of study is that usually low sample volumes are available and/or low Cu concentrations are found in these samples. This paper presents a new approach for Cu isotope ratio determination that requires only 1 μL of pre-treated serum sample per replicate (after Cu separation and preconcentration to a Cu concentration range between 0.3 and 4 mg L−1). Cu determination was carried out by direct μ-injection of 1 μL of pretreated serum samples into an ICP-MS, offering a LOD of 3 μg L−1. For Cu isotopic analysis, the method presented is based on micro-volume deposition on a pure silicon wafer and subsequent ablation analysis by fsLA-MC-ICP-MS. Cu isotopic analysis of NIST 3114 at 1 mg L−1 Cu concentration with the self-bracketing method provided average δ65Cu values of −0.01 ± 0.19‰ (2SD) and an internal precision value of 517 ppm. This method was deployed for the analysis of serum samples from WD patients under different treatments, as well as healthy newborns and patients with other liver disorders. The results seem to link decreased δ65Cu values to Cu release from the liver, further demonstrating the potential of this type of analysis in the biomedical context.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{D0JA00438C,

title = {To shift, or not to shift: adequate selection of an internal standard in mass-shift approaches using tandem ICP-mass spectrometry (ICP-MS/MS)},

author = {Eduardo Bolea-Fernandez and Ana Rua-Ibarz and Martín Resano and Frank Vanhaecke},

url = {http://dx.doi.org/10.1039/D0JA00438C},

doi = {10.1039/D0JA00438C},

year  = {2021},

date = {2021-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {36},

issue = {6},

pages = {1135-1149},

publisher = {The Royal Society of Chemistry},

abstract = {The use of an internal standard to correct for potential matrix effects and instrument instability is common practice in ICP-MS. However, with the introduction of a new generation of ICP-MS instrumentation with a tandem mass spectrometry configuration (ICP-MS/MS), the use of chemical resolution in a mass-shift approach has become much more popular, suggesting that the appropriate selection of an internal standard needs revision. In this particular case, it needs to be decided whether the internal standard should also be subjected to a mass-shift or can simply be monitored on-mass (“to shift, or not to shift”). In this work, 17 elements covering a wide range of masses (24–205 amu) and ionization energies (3.89–9.39 eV) were measured via on-mass and/or mass-shift strategies, and the corresponding atomic ions and reaction product ions were monitored during various systematic experiments. For mass-shifting, an NH3/He gas mixture was used to obtain NH3-based reaction product ions (cluster formation). Product ion scanning (PIS) was used for assessing the differences in reactivity between the different analytes and for the identification of the best suited reaction product ions. It was found that the use of chemical resolution can significantly affect the short-term signal stability and that ion signals measured on-mass are not affected in the same way as those measured mass-shifted. Variations affecting the signal intensities of both atomic and reaction product ions can be attributed to the ion–molecule chemistry occurring within the collision/reaction cell and were found to be related with some degree of initial instability in the cell and differences in reactivity. The use of a sufficiently long stabilization time, however, avoids or at least mitigates such differences in the behavior between signals monitored on-mass and after mass-shifting, respectively. Furthermore, the introduction of cell disturbances, such as those generated after quickly switching between different sets of operating conditions in a multi-tune method, revealed significant differences in signal behavior between atomic and reaction product ions, potentially hampering the use of an internal standard monitored on-mass when the analysis is based on an analyte monitored after mass-shifting. However, the use of a reasonable waiting time again greatly mitigates such differences, with the duration of this stabilization time depending on the magnitude of the cell disturbances (e.g., switch between vented and pressurized mode or only between pressurized modes using different gas flow rates). In addition, also the effect of varying different instrument settings (plasma power, torch position, and gas and liquid flow rates) was evaluated, but no remarkable differences were found between signals monitored on-mass and those mass-shifted. Interestingly, a statistical evaluation of the influence of the different settings on the signal intensities of all ions monitored did not reveal the a priori important role of some properties traditionally suggested for adequate selection of analyte/internal standard pairs, such as mass number or ionization energy, as also suggested in other recent studies.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{BORQUEFERNANDO20218,

title = {Genetic counseling in prostate cancer: How to implement it in daily clinical practice?},

author = {A. Borque-Fernando and R. Espílez and D. Miramar and D. Corbatón and A. Rodríguez and E. Castro and J. Mateo and L. Rello and A. Méndez and M. J. Gil Sanz},

url = {https://www.sciencedirect.com/science/article/pii/S2173578620301566},

doi = {https://doi.org/10.1016/j.acuroe.2020.08.010},

issn = {2173-5786},

year  = {2021},

date = {2021-01-01},

journal = {Actas Urológicas Españolas (English Edition)},

volume = {45},

number = {1},

pages = {8-20},

abstract = {Prostate cancer plays an undeniably prominent role in public health in our days and health systems. Its epidemiological impact is quantitatively very close to that of other tumors such as colon cancer and breast cancer, in which genetic counseling is part of their routine clinical practice, both in the initial evaluation and in the selection of therapeutic strategies. Hereditary cancer syndromes, breast/ovarian and Lynch syndrome are part of genetic counseling in these tumors. Currently, we also know that they can be associated to prostate cancer. The time has come to implement genetic counseling in prostate cancer from the earliest stages of its approach, from initial suspicion to the most advanced tumors. We present an updated review carried out by our interdisciplinary working group on scientific literature, clinical practice guidelines and consensus documents, aimed at the creation and drafting of a ‘Protocol for genetic counseling in prostate cancer’ for the study of germline, with easy application in different healthcare settings. This protocol is currently being implemented in our routine practice and provides answers to 3 specific questions: Who should receive genetic counseling for prostate cancer? Which gene panel should be analyzed? How should counseling be done according to the results obtained? Other aspects about who should perform genetic counseling, ethical considerations and regulations are also collected. 

Resumen 

El cáncer de próstata tiene un protagonismo socio-sanitario innegable en nuestros días y sistemas de salud. Su impacto epidemiológico cuantitativamente está muy próximo a otros tumores como el cáncer de colon y el cáncer de mama, en los que el asesoramiento genético forma parte de su práctica clínica habitual, tanto en la evaluación inicial como en la selección de estrategias terapéuticas. Los síndromes de cáncer hereditario, mama/ovario y síndrome de Lynch, forman parte del asesoramiento genético en estos tumores y hoy día también sabemos que pueden tener relación con el cáncer de próstata. Ha llegado el momento de implementar el asesoramiento genético en cáncer de próstata desde las etapas más iniciales de su abordaje, desde la sospecha inicial hasta los tumores más avanzados.Presentamos una revisión actualizada de nuestro grupo de trabajo interdisciplinar sobre la literatura científica, guías de práctica clínica y documentos de consenso hasta la creación y redacción de un «Protocolo de asesoramiento genético en cáncer de próstata», centrado en el estudio de línea germinal, de fácil aplicabilidad en los diferentes entornos asistenciales. Dicho protocolo se encuentra actualmente implementado en nuestra práctica habitual y da respuesta a tres preguntas concretas: ¿A quién realizar asesoramiento genético en cáncer de próstata?, ¿qué panel de genes analizar?, y ¿cómo aconsejar de acuerdo con los resultados obtenidos? Otros aspectos acerca de quién debe realizar el asesoramiento genético, consideraciones éticas y normativa también son recogidos.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32745579,

title = {Serum protein profile analysis in lysosomal storage disorders patients},

author = {Laura López de Frutos and Elena García-González and Beatriz García-Rodríguez and Yolanda González-Irazabal and Carlos Lahoz and Pilar Irún and Jorge J Cebolla and Pilar Giraldo},

doi = {10.1016/j.cca.2020.07.056},

issn = {1873-3492},

year  = {2020},

date = {2020-11-01},

journal = {Clin Chim Acta},

volume = {510},

pages = {430--436},

abstract = {INTRODUCTION: Serum protein electrophoresis (SPE) is a well-established technique to identify alterations in plasma protein profiles, caused by diseases as multiple myeloma (MM). In addition, it could be a cost-effective technique to discover new plasma biomarkers. Relation between MM and lysosomal storage diseases (LSDs) as Gaucher disease has been set out but, it has not been evaluated on other LSDs nor the utility of the SPE as first step on LSDs biomarkers discovery projects.



MATERIALS AND METHODS: Stored plasma samples at diagnosis from several LSDs patients underwent analysis. Quality control was checked prior to the SPE was analyzed by capillary electrophoresis. The analysis for monoclonal spikes and the differences between each fraction on patients' samples vs the control data previously published, were evaluated. Furthermore, immunoprotein quantification and free light chains ratio were done by nephelometry and turbidimetry.



RESULTS: Seventy-five samples of LSD patients at diagnosis, were assessed. The frequency of the MGUS on LSDs patients was not higher than in general population whereas one lysosomal acid lipase deficiency infant showed increased IgA and kappa deviation. Regarding to the usefulness of SPE in biomarkers discovery, statistically significant differences were observed on SPE fractions between LSDs and healthy population.



DISCUSSION: The evaluation of SPE fractions can be a useful tool to understand pathophysiologic aspects in LDSs and, to simplify new marker discovery projects. In some of them, the MGUS appearance is a risk factor for the MM development despite its frequency is not increased on the studied LSDs at diagnosis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32314013,

title = {Contributions to the study of spinocerebellar ataxia type 38 (SCA38)},

author = {José Gazulla and Elvira Orduna-Hospital and Isabel Benavente and Ana Rodríguez-Valle and Pedro Osorio-Caicedo and Sara Alvarez-de Andrés and Elena García-González and Jesús Fraile-Rodrigo and Francisco Javier Fernández-Tirado and José Berciano},

doi = {10.1007/s00415-020-09840-1},

issn = {1432-1459},

year  = {2020},

date = {2020-08-01},

journal = {J Neurol},

volume = {267},

number = {8},

pages = {2288--2295},

abstract = {OBJECTIVE: To report clinical and ancillary findings in a kindred with spinocerebellar ataxia 38 (SCA38).



PATIENTS AND METHODS: Five family members spanning two generations developed gait ataxia and intermittent diplopia. On examination, a cerebellar syndrome accompanied by downbeat nystagmus and a saccadic head impulse test (HIT) were found.



RESULTS: Whole-exome sequencing demonstrated a heterozygous variant in ELOVL5, c.779A > G (p.Tyr260Cys), in four tested patients. Intermittent concomitant esotropia and hypertropia caused transient diplopia in one individual each. Saccadic HIT responses were found in four subjects. Sensorineural hypoacusis was present in every case. Electrophysiological studies demonstrated a sensory neuronopathy in patients from the first generation, with prolonged disease duration. Baseline serum docosahexaenoic acid (DHA) percent was diminished in four individuals. Oral 26-week dietary DHA supplementation, 650 mg/day, raised serum DHA percent and induced a statistically significant reduction in Scale for the Assessment and Rating of Ataxia (SARA) total scores, and in stance and heel-shin slide item scores.



CONCLUSION: The mentioned ELOVL5 variant segregated with disease in this kindred. Downbeat nystagmus, intermittent heterotropia causing transient diplopia, vestibular impairment demonstrated by abnormal HIT, and sensory neuronopathy were part of the clinical picture in this series. DHA supplementation raised serum DHA percent in cases with diminished levels, and induced a clinical amelioration and a statistically significant reduction in SARA scores in the study group. Further studies are needed to investigate the role of these findings in SCA38, and to determine the response to prolonged DHA supplementation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RESANO2020115955,

title = {Breaking the boundaries in spectrometry. Molecular analysis with atomic spectrometric techniques},

author = {Martín Resano and Maite Aramendía and Flávio V. Nakadi and Esperanza García-Ruiz and César Alvarez-Llamas and Nerea Bordel and Jorge Pisonero and Eduardo Bolea-Fernández and Tong Liu and Frank Vanhaecke},

url = {https://www.sciencedirect.com/science/article/pii/S0165993620301849},

doi = {https://doi.org/10.1016/j.trac.2020.115955},

issn = {0165-9936},

year  = {2020},

date = {2020-01-01},

journal = {TrAC Trends in Analytical Chemistry},

volume = {129},

pages = {115955},

abstract = {Since the development of atomic spectrometry, trace element and isotopic analysis has been mainly based on the monitoring of atomic spectra and monoionic species. However, according to the literature and considering the current instrumental developments, it seems that some of the remaining challenges in this field can be mitigated via the measurement of molecular spectra or of polyatomic ions. This review discusses recent advances in three of the most important atomic techniques (laser-induced breakdown spectrometry, high-resolution continuum source atomic absorption spectrometry and inductively coupled plasma mass spectrometry) and how the monitoring of such molecules or polyatomic ions containing the target analyte enables attaining better selectivity and opens new ways to determine non-metals and to obtain isotopic information.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{D0JA00359J,

title = {Introducing multi-energy ratios as an alternative to multi-energy calibration for Br determination via high-resolution continuum source graphite furnace molecular absorption spectrometry. A case study},

author = {Raúl Garde and Flávio V. Nakadi and Esperanza García-Ruiz and Martín Resano},

url = {http://dx.doi.org/10.1039/D0JA00359J},

doi = {10.1039/D0JA00359J},

year  = {2020},

date = {2020-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {35},

issue = {11},

pages = {2606-2619},

publisher = {The Royal Society of Chemistry},

abstract = {This manuscript explores the advantages of using multi-signal calibration approaches for the determination of non-metals via high-resolution continuum source graphite furnace molecular absorption spectrometry (HR CS GFMAS), targeting Br as an example. Besides multi-energy calibration (MEC), a novel approach deriving from it, multi-energy ratios (MER), is introduced and compared under different conditions. This approach makes use of the same data but in a different way, such that no linear regression is performed; instead, ratios are calculated. This article investigates the potential errors deriving from the use of amounts of spike dissimilar from the sample content, leading to too high (close to 1) or too low (close to 0) slopes/ratios, setting the best conditions in terms of precision and accuracy for the intended determination in the range of approx. 0.5 to 0.6. Also, situations where the use of MER could be recommended over MEC are identified: namely when only a few transitions of sufficient sensitivity and free from overlaps are available or else, many transitions but of similar sensitivity, which may occur when HR CS GFMAS is deployed. Otherwise, for multiple transitions covering a wider sensitivity range, use of linear regression and thus, of MEC, seems favoured, as a better precision can be achieved. The calculation of limits of detection and quantification for both approaches is also discussed. It is finally further demonstrated that these multi-signal strategies help in solving chemical interferences, which very often hamper the determination of non-metals with HR CS GFMAS, and they do so in a simple way, without the need for laborious work or for the preparation of several standards and sample aliquots, therefore making them a very intriguing option when this technique is deployed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{D0JA00254B,

title = {Understanding polyatomic interference in the determination of phosphorus via PO molecules using high-resolution continuum source graphite furnace molecular absorption spectrometry with direct solid analysis},

author = {Luiza Carolina Pomarolli and Márcia Andréia Mesquita Silva Veiga and Martín Resano and Flávio Venâncio Nakadi},

url = {http://dx.doi.org/10.1039/D0JA00254B},

doi = {10.1039/D0JA00254B},

year  = {2020},

date = {2020-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {35},

issue = {10},

pages = {2305-2314},

publisher = {The Royal Society of Chemistry},

abstract = {This study describes the occurrence of a molecular interference affecting the determination of phosphorus by the molecular absorption of phosphorus monoxide (PO) at 213.647 nm using high-resolution continuum source graphite furnace molecular absorption spectrometry with direct solid sample analysis (HR CS SS-GF MAS). The study shows how the observation of the background signal provided by the software, which typically goes unnoticed as it is not well understood what is represented by it, provides valuable information for method development. In this case, the background signal hints at the formation of a concomitant polyatomic molecule in all solid samples investigated that is more volatile than PO and that can cause disturbances in the absorption of the target molecule. This information is paramount to properly optimize the temperature program. The most effective modifier to achieve quantitative PO molecule formation and better thermal stability consisted of a mixture of Au and Ca. The pyrolysis temperature was set at 1500 °C to minimize the influence of the interference mentioned above. The vaporization temperature was set at 2200 °C. Following this procedure, certified reference materials of leaf samples (NIST 1547, NIST 1570a, and NIST 1573a) could be accurately analyzed simply using external calibration with P aqueous solutions. Macauba and eucalyptus real samples were also analyzed, showing P concentrations of 455 ± 39 and 400 ± 35 μg g−1, respectively. The limit of detection of the method was found to be 0.078 μg (n = 5), which translates into 78 μg g−1 when using one mg of the sample, which seems appropriate for this type of sample.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{C9JA00348G,

title = {A simple and direct atomic absorption spectrometry method for the direct determination of Hg in dried blood spots and dried urine spots prepared using various microsampling devices},

author = {Flávio V. Nakadi and Raúl Garde and Márcia A. M. S. Veiga and Julio Cruces and Martín Resano},

url = {http://dx.doi.org/10.1039/C9JA00348G},

doi = {10.1039/C9JA00348G},

year  = {2020},

date = {2020-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {35},

issue = {1},

pages = {136-144},

publisher = {The Royal Society of Chemistry},

abstract = {The use of dried matrix spots, such as dried blood spots (DBSs) and dried urine spots (DUSs), is becoming more popular in clinical analyses, beyond their traditional use for screening newborns. Currently, there are new types of microsampling devices that have been designed to collect a low and known volume of biological fluids, regardless of the hematocrit level, thus making it feasible to develop fully quantitative methods for their analysis. In this study, three of the most promising microsampling devices (Capitainer B, Hemaxis DB 10 and Mitra) were evaluated aiming at the direct determination of Hg using a dedicated “analyzer” based on atomic absorption spectrometry. Whole blood and urine reference materials were used to evaluate the methods developed. This Hg analyzer possesses a gold trap to enhance the sensitivity and minimize matrix effects, enabling direct analysis of the solid DBS and DUS samples using calibration with Hg aqueous solutions, with an instrumental limit of detection (LOD) and quantification (LOQ) of 3.4 and 11 pg, respectively. LODs when using the three microsampling devices were similar, ranging between 2.5 and 3.2 μg L−1. It is also demonstrated that the simultaneous analysis of four Mitra derived DBSs in a quartz boat results in a decrease of the LOD to 0.32 μg L−1.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{VICENTINO2020120230,

title = {Extraction induced by microemulsion breaking as a novel tool for the simultaneous determination of Cd, Mn, Pb and Sb in gasoline samples by ICP-MS and discrete sample introduction},

author = {Priscila O. Vicentino and Ricardo J. Cassella and Diego Leite and Martín Resano},

url = {https://www.sciencedirect.com/science/article/pii/S003991401930863X},

doi = {https://doi.org/10.1016/j.talanta.2019.120230},

issn = {0039-9140},

year  = {2020},

date = {2020-01-01},

journal = {Talanta},

volume = {206},

pages = {120230},

abstract = {This study reports a simple and efficient method for the simultaneous determination of Cd, Mn, Pb, and Sb at trace levels in gasoline samples by discrete sample introduction in inductively coupled plasma mass spectrometry (ICP-MS), employing the extraction induced by microemulsion breaking as a sample preparation method. In this work, a microemulsion was formed by mixing 0.7 mL of the gasoline sample with 0.28 mL of ethanol and 0.02 mL of 7 mol L−1 HNO3 solution. Afterwards, 0.1 mL of ultrapure water was added to induce microemulsion breaking. This lead to the formation of two different phases: (i) a top organic phase and (ii) a bottom aqueous phase that contained acid, ethanol and the extracted analytes. After that, 20 μL of the bottom phase (extract) was collected, and the analyte concentrations were determined by discrete sample introduction in ICP-MS. Calibration curves with 20 μg L−1 of Rh as an internal standard were set up to each analyte in the range from 1 up to 10 μg L−1, wherein the standard solutions were prepared in the matrix-matched extract (70:25:5, ethanol: deionized water: 7 mol L−1 HNO3 solution). The optimization of the EIMB conditions was carried out by analyzing the effects of the parameters that could affect the extraction efficiency, such as the relation between the nature of the alcohol and the proportion of the volumes of the microemulsion constituents (sample, HNO3 and alcohol), acid concentration, and the water volume used for the microemulsion breaking. The following limits of detection relative to the amount of sample were obtained: 0.03 (Cd), 0.49 (Mn), 0.07 (Pb) and 0.02 (Sb), all of them in μg L−1. The accuracy of the method was evaluated by the analysis of spiked samples, because no certified gasoline reference material is available. The average recoveries achieved for every analyte ranged from 96% to 114%. The developed methodology was successfully applied to the determination of these metals in five commercial gasoline samples.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31637781,

title = {Prevalence of FLG loss-of-function mutations R501X, 2282del4, and R2447X in Spanish children with atopic dermatitis},

author = {Ricardo González-Tarancón and Rosalia Sanmartín and Fabiola Lorente and Elvira Salvador-Rupérez and Angela Hernández-Martín and Luis Rello and Jose Puzo and Yolanda Gilaberte},

doi = {10.1111/pde.14025},

issn = {1525-1470},

year  = {2020},

date = {2020-01-01},

journal = {Pediatr Dermatol},

volume = {37},

number = {1},

pages = {98--102},

abstract = {BACKGROUND/OBJECTIVES: Atopic dermatitis (AD) is the most prevalent inflammatory skin disorder, and is often associated with a personal or family history of atopic disease. The presence of loss-of-function mutations in the filaggrin gene (FLG) is the main predisposing factor for AD FLG mutations show ethnic and geographical variations, even between European populations. We sought to determine the frequency of the 3 most common FLG null mutations in a population of Spanish children consisting of healthy controls and AD patients. We also investigated the association between these 3 FLG mutations and AD.



METHODS: A total of 214 participants (111 AD patients and 103 healthy controls) were enrolled in this study. Genotyping for 3 FLG null mutations (R501X, 2282del4, and R2447X) was performed by conventional Sanger sequencing.



RESULTS: The combined mutation frequency was 1.9% in the control group and 12.6% in the AD group. The most common FLG mutation in AD patients was R501X (9.9%), followed by R2447X (2.7%) and 2282del4 (1.8%).



CONCLUSION: These findings further our understanding of the prevalence of FLG null mutations in the Spanish population, and suggest that the frequency of FLG mutations in AD patients in Spain is slightly higher than that of other Mediterranean countries.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{BOLEAFERNANDEZ201995,

title = {On the effect of using collision/reaction cell (CRC) technology in single-particle ICP-mass spectrometry (SP-ICP-MS)},

author = {Eduardo Bolea-Fernandez and Diego Leite and Ana Rua-Ibarz and Tong Liu and Glenn Woods and Maite Aramendia and Martín Resano and Frank Vanhaecke},

url = {https://www.sciencedirect.com/science/article/pii/S0003267019306920},

doi = {https://doi.org/10.1016/j.aca.2019.05.077},

issn = {0003-2670},

year  = {2019},

date = {2019-01-01},

journal = {Analytica Chimica Acta},

volume = {1077},

pages = {95-106},

abstract = {In this work, the effects of using collision/reaction cell (CRC) technology in quadrupole-based ICP-MS (ICP-QMS) instrumentation operated in single-particle (SP) mode have been assessed. The influence of (i) various CRC gases, (ii) gas flow rates, (iii) nanoparticle (NP) sizes and (iv) NP types was evaluated using Ag, Au and Pt NPs with both a traditional ICP-QMS instrument and a tandem ICP-mass spectrometer. It has been shown that using CRC technology brings about a significant increase in the NP signal peak width (from 0.5 up to 6 ms). This effect is more prominent for a heavier gas (e.g., NH3) than for a lighter one (e.g., H2 or He). At a higher gas flow rate and/or for larger particle sizes >100 nm), the NP signal duration was prolonged to a larger extent. This effect of using CRC technology has been further demonstrated by characterizing custom-made 50 and 200 nm Fe3O4 NPs (originally strongly affected by the occurrence of spectral overlap) using different CRC approaches (H2 on-mass and NH3 mass-shift). The use of NH3 (monitoring of Fe as the Fe(NH3)2+ reaction product ion at m/z = 90 amu) induces a significant peak broadening compared to that observed when using H2 (6.10 ± 1.60 vs. 0.94 ± 0.49 ms). This extension of transit time can most likely be attributed to the collisions/interactions of the ion cloud generated by a single NP event with the CRC gas and it even precludes 50 nm Fe3O4 NPs to be detected when using the NH3 mass-shift approach. Based on these results, the influence of a longer peak width on the accuracy of SP-ICP-MS measurement data (NP size, particle number density and mass concentration) must be taken into account when using CRC technology as a means to overcome spectral overlap. To mitigate the potential detrimental effect of using CRC technology in the characterization of NPs via SP-ICP-MS(/MS), the use of light gases and low gas flow rates is recommended.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MARGUI20197,

title = {A sustainable and simple energy dispersive X-ray fluorescence method for sulfur determination at trace levels in biodiesel samples via formation of biodiesel spots on a suitable solid support},

author = {Eva Marguí and Martín Resano and Ignasi Queralt},

url = {https://www.sciencedirect.com/science/article/pii/S0584854718305561},

doi = {https://doi.org/10.1016/j.sab.2019.04.003},

issn = {0584-8547},

year  = {2019},

date = {2019-01-01},

journal = {Spectrochimica Acta Part B: Atomic Spectroscopy},

volume = {156},

pages = {7-12},

abstract = {The aim of the present work is the development of a simple, sensitive and sustainable EDXRF method for the determination of trace amounts of sulfur in biodiesel samples. In this method, the deposition of several microliters of sample onto an organic thin layer and the analysis of the resulting adsorbed biodiesel spot by benchtop EDXRF is proposed. A careful study was performed to select the volume and the best solid support to deposit biodiesel samples, including filters made of different materials (glass fiber, Nylon, cellulose, paper) and a commercial disposable absorbent pad (UltraCarry, Rigaku). A critical issue that limits the use of most of these solid supports was the relative high blank signals that hamper the determination of sulfur at trace levels. Finally, it was found that best strategy was the deposition of 50 μL of biodiesel on the UltraCarry sample retainer. Operating conditions for EDXRF measurements were also evaluated to obtain the best instrumental sensitivity for sulfur determination (Excitation: 20 kV, no primary filter, measurement time: 300 s). Using the best analytical conditions the quantification limit of the method was 7 mg kg−1 of sulfur. This value is even better than the one reported in the ASTM D4294 method (LOQ: 16.0 mg kg−1) but using a sample amount 100 times smaller. The linearity was confirmed in the range of 10–100 mg kg−1by analyzing a set of commercial biodiesel standards. Accuracy and precision of the results, evaluated by the analysis of samples prepared with the same matrix as the standards, with levels of 20, 40 and 75 mg kg−1of sulfur, and processed as unknowns, proved acceptable (Recoveries: 94.3–110.6%, RSD: 10.8–13.6%},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{C9JA00233B,

title = {Calcium isotope determination in urine samples via the monitoring of 44CaF and 40CaF molecules by high-resolution continuum source graphite furnace molecular absorption spectrometry},

author = {Melina B. T. Zanatta and Flávio V. Nakadi and Martin Resano and Márcia A. M. S. Veiga},

url = {http://dx.doi.org/10.1039/C9JA00233B},

doi = {10.1039/C9JA00233B},

year  = {2019},

date = {2019-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {34},

issue = {11},

pages = {2280-2287},

publisher = {The Royal Society of Chemistry},

abstract = {This study investigates the possibility of obtaining Ca isotopic information by monitoring the absorption spectrum of a diatomic molecule generated in a graphite furnace using a high-resolution continuum source spectrometer (HR CS GFMAS). The method proposed enables calcium isotopic analysis using CaF as the target species. The difference in wavelength between 40CaF (maximum at 628.534 nm) and 44CaF (maximum at 628.191 nm) absorption signals was found to be 337.8 pm, evidencing that both species can be determined simultaneously. The method developed relies on the determination of 40Ca and 44Ca concentrations based on calibration functions specific to each isotope and makes use of 12 μg of F as a molecule forming element without further chemical modifiers. The sensitivity of the method depends on the pH. It increases at pH values close to 2, and decreases until reaching a plateau at pH 6. The characteristic mass found for 40CaF was 0.11 μg for solutions with pH 2 and 0.18 μg for alkaline solutions. The respective limits of detection were 1 and 2 mg L−1. The method was applied to the analysis of three urine samples, spiked with different 40Ca and 44Ca amounts. Due to chlorine interference, before the analysis of urine, calcium must be separated from the samples by precipitation with ammonium oxalate. Recovery tests provided results between 95 and 109%, highlighting the potential of the method for 40Ca and 44Ca determination in tracer experiments.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MARGUI20197b,

title = {A sustainable and simple energy dispersive X-ray fluorescence method for sulfur determination at trace levels in biodiesel samples via formation of biodiesel spots on a suitable solid support},

author = {Eva Marguí and Martín Resano and Ignasi Queralt},

url = {https://www.sciencedirect.com/science/article/pii/S0584854718305561},

doi = {https://doi.org/10.1016/j.sab.2019.04.003},

issn = {0584-8547},

year  = {2019},

date = {2019-01-01},

journal = {Spectrochimica Acta Part B: Atomic Spectroscopy},

volume = {156},

pages = {7-12},

abstract = {The aim of the present work is the development of a simple, sensitive and sustainable EDXRF method for the determination of trace amounts of sulfur in biodiesel samples. In this method, the deposition of several microliters of sample onto an organic thin layer and the analysis of the resulting adsorbed biodiesel spot by benchtop EDXRF is proposed. A careful study was performed to select the volume and the best solid support to deposit biodiesel samples, including filters made of different materials (glass fiber, Nylon, cellulose, paper) and a commercial disposable absorbent pad (UltraCarry, Rigaku). A critical issue that limits the use of most of these solid supports was the relative high blank signals that hamper the determination of sulfur at trace levels. Finally, it was found that best strategy was the deposition of 50 μL of biodiesel on the UltraCarry sample retainer. Operating conditions for EDXRF measurements were also evaluated to obtain the best instrumental sensitivity for sulfur determination (Excitation: 20 kV, no primary filter, measurement time: 300 s). Using the best analytical conditions the quantification limit of the method was 7 mg kg−1 of sulfur. This value is even better than the one reported in the ASTM D4294 method (LOQ: 16.0 mg kg−1) but using a sample amount 100 times smaller. The linearity was confirmed in the range of 10–100 mg kg−1by analyzing a set of commercial biodiesel standards. Accuracy and precision of the results, evaluated by the analysis of samples prepared with the same matrix as the standards, with levels of 20, 40 and 75 mg kg−1of sulfur, and processed as unknowns, proved acceptable (Recoveries: 94.3–110.6%, RSD: 10.8–13.6%},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ALVAREZBALLANO201921,

title = {Intervalos de referencia de hormonas tiroideas en mujeres gestantes mediante 2 inmunoanálisis diferentes: la importancia del método por encima de valores únicos universales, en consonancia con las recomendaciones internacionales 2017},

author = {D. Álvarez Ballano and M. O. Bandrés Nivela and M. L. Gracia Ruiz and A. Ilundain González and P. Diego García and Y. Blasco Lamarca and A. B. Mañas Martínez and E. García-González and L. Rello Varas and M. A. Sancho Serrano and J. J. Puente Lanzarote},

url = {https://www.sciencedirect.com/science/article/pii/S0210573X17300588},

doi = {https://doi.org/10.1016/j.gine.2017.09.001},

issn = {0210-573X},

year  = {2019},

date = {2019-01-01},

journal = {Clínica e Investigación en Ginecología y Obstetricia},

volume = {46},

number = {1},

pages = {21-27},

abstract = {Resumen 

Antecedentes y objetivos 

La disfunción tiroidea durante la gestación repercute sobre la salud materno-fetal y puede influir en el desarrollo neurocognitivo del niño. La fisiología tiroidea cambia en el embarazo y obliga a establecer valores de referencia (VR) para cada población y método. Los objetivos fueron determinar dichos VR de hormonas tiroideas (HT) empleando 2 inmunoanálisis, estimar el estado nutricional de yodo y la prevalencia de autoinmunidad tiroidea en nuestra población. 

Pacientes y métodos 

Se seleccionó a 378 gestantes de los sectores sanitarios de Zaragoza y Huesca, con determinación de yoduria, anticuerpos antitiroideos y HT mediante 2 inmunoanálisis diferentes (Beckman y Siemens). 

Resultados 

Yoduria media 187μg/L, mediana 146μg/L. El 78% tomaba suplemento (yoduro potásico) y su consumo se relacionó con mayores niveles de yoduria. El 10,8% tenían anticuerpos antiperoxidasa positivos, el 4,4% antitiroglobulina, el 2,4% ambos y el 4,1% anti-TSHr. No hubo asociación entre yoduria y TSH ni T4L. Los VR de TSH en el primer trimestre fueron Beckman 0,2-4 y Siemens 0,2-3,4 mUI/L. 

Conclusión 

Los VR de HT fueron claramente diferentes a los propuestos por la ATA 2011 pero prácticamente iguales a los descritos en población española utilizando los mismos inmunoanálisis, como propone la ATA 2017. La autoinmunidad tiroidea fue similar a la publicada a nivel nacional e internacional. La media y la mediana de yodurias son de las más elevadas publicadas en España hasta el momento y dependen principalmente de la toma de yoduro potásico para alcanzar los objetivos de la OMS, lo que avala las recomendaciones de suplementación con al menos 150μg de yodo. 

Background and objectives 

Thyroid dysfunction during pregnancy affects maternal and foetal health, which may influence the child's neurocognitive development. The thyroid physiology changes during pregnancy, requiring reference values (RV) to be established for each population and method. The objectives were to determine these thyroid hormone (TH) RV using 2 immunoassays and to estimate the nutritional status of iodine and the prevalence of thyroid autoimmunity in our population. 

Patients and methods 

A total of 378 pregnant women from the health sectors of Zaragoza and Huesca, whose urinary iodine, antithyroid antibody and TH levels were assessed by 2different immunoassays (Beckman and Siemens), were enrolled. 

Results 

The mean urinary iodine concentration was 187μg/l, with a median concentration of 146μg/l. From them, 78% took potassium iodide supplements and their consumption was related to higher levels of urinary iodine; 10.8% were positive for antithyroid peroxidase antibodies, 4.4% for anti-thyroglobulin antibodies, 2.4% for both and 4.1% for anti-TSHr. There was no association between urinary iodine and TSH or T4L. The reference values of TSH in the first trimester were Beckman: 0.2-4 and Siemens 0.2-3.4 mIU/l. 

Conclusion 

The thyroid hormone reference values were markedly different from those proposed by the ATA-2011 guidelines but practically identical to those described in the Spanish population using the same immunoassays, as proposed by the ATA-2017 guidelines. Thyroid autoimmunity was similar to that published nationally and internationally. The mean and median urinary iodine levels are among the highest published in Spain to date and depend mainly on supplementation with potassium iodide to reach the WHO objectives, supporting the recommendations for supplementation with at least 150μg of iodine.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{C8JA00256H,

title = {Quo vadis high-resolution continuum source atomic/molecular absorption spectrometry?},

author = {M. Resano and E. García-Ruiz and M. Aramendía and M. A. Belarra},

url = {http://dx.doi.org/10.1039/C8JA00256H},

doi = {10.1039/C8JA00256H},

year  = {2019},

date = {2019-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {34},

issue = {1},

pages = {59-80},

publisher = {The Royal Society of Chemistry},

abstract = {After more than a decade since its commercial introduction, high-resolution continuum source atomic/molecular absorption spectrometry may be facing a mid-life crisis. Certainly, it is no longer a novel technique full of unknown potential, so it would already be time to establish the fields for which it is most suitable. This is, however, not so simple for a number of reasons. In the first place, more than a technique what we are discussing herein is a type of instrumentation with the potential to use two different techniques (atomic or molecular absorption), making it somewhat unique. Furthermore, the two techniques have not been explored equally, and more research on the mechanisms of formation of diatomic molecules is clearly needed. In the second place, new possibilities have recently appeared in the literature that need to be weighed as well. And there is the still unfulfilled, but nowadays more technically feasible than ever, promise to significantly increase the multi-elemental capabilities. This review critically examines the main research areas currently explored (namely, (i) direct analysis of solids and complex liquid materials, and (ii) determination of non-metals at trace levels via monitoring of molecular species) as well as the new venues (specifically, (i) isotopic analysis via monitoring of molecular species, and (ii) selective detection, quantification and sizing of nanoparticles) while also considering new instrumental developments, in an attempt to properly place high-resolution continuum source atomic/molecular absorption spectrometry in the field of trace element and isotopic analysis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}