Publications

@article{C2JA30262D,

title = {Direct determination of Cu isotope ratios in dried urine spots by means of fs-LA-MC-ICPMS. Potential to diagnose Wilson's disease},

author = {Martín Resano and Maite Aramendía and Luis Rello and Mª Luisa Calvo and Sylvain Bérail and Christophe Pécheyran},

url = {http://dx.doi.org/10.1039/C2JA30262D},

doi = {10.1039/C2JA30262D},

year  = {2013},

date = {2013-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {28},

issue = {1},

pages = {98-106},

publisher = {The Royal Society of Chemistry},

abstract = {This work investigates the potential of a 257 nm femtosecond (fs) laser ablation (LA) device operating at a high repetition rate (10 000 Hz) coupled to a multicollector (MC)-ICPMS to develop a method for the direct determination of Cu ratios in dried urine spots, prepared by deposition of urine (300 μL) onto precut clinical filter paper discs (16 mm diameter). The sampling capabilities offered by the fs LA system, permitting ablation of 150 μm thick coronas in the rim area of the filter, together with the use of admixed Ni as an internal standard, the proper optimization of the MC-ICPMS conditions (e.g., use of pseudo high-resolution mode to avoid interferences) and the use of a data processing approach particularly suitable for short transient signals (linear regression fit) enabled analysis of real urine samples with precision values around 500 ppm (RSD) for urinary Cu contents of a few hundred μg L−1. The methodology developed could prove to be useful for implementing screening protocols to detect Wilson's disease (WD), a well-known disorder related to Cu metabolism. In fact, the use of this analytical methodology permitted us to observe significant differences between (i) untreated WD patients and (ii) WD patients that are under treatment and control samples. This work represents the first time that determination of 65Cu/63Cu ratios has been used in the context of WD research, and serves as a proof of principle, suggesting that Cu isotope analysis could help in developing earlier and more reliable means to diagnose WD.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{C2JA30265A,

title = {Accurate determination of S in organic matrices using isotope dilution ICP-MS/MS},

author = {Lieve Balcaen and Glenn Woods and Martín Resano and Frank Vanhaecke},

url = {http://dx.doi.org/10.1039/C2JA30265A},

doi = {10.1039/C2JA30265A},

year  = {2013},

date = {2013-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {28},

issue = {1},

pages = {33-39},

publisher = {The Royal Society of Chemistry},

abstract = {Accurate determination of low levels of S in organic matrices by means of isotope dilution ICP-single quadrupole MS (e.g., for quantification of S components in reverse phase HPLC-ICP-MS experiments) is often not feasible. This work describes an accurate, sensitive and fast analytical method for the determination of S in organic matrices by means of a recently developed ‘triple quadrupole’ ICP-MS instrument, operated in MS/MS mode. The added value of the MS/MS approach for this application has been clearly visualised by varying the width of the bandpass of the first quadrupole analyzer from “fully open” (standard mode) down to single mass width (MS/MS mode). As a proof-of-concept, a biodiesel reference material has been analysed for its S content with the proposed method, using isotope dilution for calibration, and the results obtained were in excellent agreement with the certified value (within experimental uncertainty).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{C3JA30349G,

title = {Isotopic analysis of Cu in serum samples for diagnosis of Wilson's disease: a pilot study},

author = {Maite Aramendía and Luis Rello and Martín Resano and Frank Vanhaecke},

url = {http://dx.doi.org/10.1039/C3JA30349G},

doi = {10.1039/C3JA30349G},

year  = {2013},

date = {2013-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {28},

issue = {5},

pages = {675-681},

publisher = {The Royal Society of Chemistry},

abstract = {Wilson's disease (WD) is a genetic disorder affecting Cu metabolism, which can lead to severe physiological and neurological symptoms, and even death if untreated. Based on the fact that WD patients show low Cu levels in serum, implementation of screening programs for diagnosis of this condition at the moment of birth, when progression of the disease can be still arrested, has been attempted in the past. These attempts, however, have been unsuccessful, as healthy new-borns often show low Cu levels in serum due to liver immaturity. In this work, the potential use of isotopic analysis of Cu in serum samples as an alternative diagnostic parameter for Wilson's disease has been investigated. For this purpose, the Cu isotopic composition of a set of serum samples from different groups showing either low (i.e. WD patients, patients who had undergone bariatric surgery, infants) or normal (supposedly healthy adults) Cu concentration levels was determined by means of multi-collector ICP-mass spectrometry (MC-ICP-MS), after chromatographic isolation of Cu. For this purpose, AG-MP-1 strong anion exchange resin was relied upon, enabling quantitative recovery of Cu in pure form from the serum samples. MC-ICP-MS measuring conditions were optimized to avoid the influence of spectral overlap, and Ni was admixed as an internal standard for correction of instrumental mass discrimination. The use of this optimized method provided δ65Cu for the serum samples with a typical analytical uncertainty of ±0.20‰ (k = 2). Our results show that, for the population considered in this study, combination of Cu concentration values and Cu isotopic information allows classification of WD patients, infants and controls into different groups, while the use of concentration values only is not sufficient for this purpose. Although further studies with a larger number of samples are needed, results are encouraging as far as the use of Cu isotopic analysis for early diagnosis of Wilson's disease is concerned.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid22907558,

title = {Mineral concentrations in hair of Belgian elementary school girls: reference values and relationship with food consumption frequencies},

author = {Barbara Vanaelst and Inge Huybrechts and Nathalie Michels and Krishna Vyncke and Isabelle Sioen and Tineke De Vriendt and Maria R Flórez and Maite Aramendía and Lieve Balcaen and Martin Resano and Frank Vanhaecke and Stefaan De Henauw},

doi = {10.1007/s12011-012-9495-5},

issn = {1559-0720},

year  = {2012},

date = {2012-12-01},

journal = {Biol Trace Elem Res},

volume = {150},

number = {1-3},

pages = {56--67},

abstract = {Although evidence suggests that hair elements may reflect dietary habits and/or mineral intake, this topic remains controversial. This study therefore presents age-specific reference values for hair concentrations of Ca, Cu, Fe, Na, Mg, P and Zn using the LMS method of Cole, and investigates the relationship between dietary habits (i.e. food consumption frequencies) and hair mineral concentrations in 218 Belgian elementary school girls by reduced rank regression (RRR). Hair minerals were quantitatively determined via inductively coupled plasma-mass spectrometry after microwave-assisted acid digestion of 6-cm long vertex posterior hair samples. The Children's Eating Habits Questionnaire-Food Frequency Questionnaire was used to obtain information on food consumption frequency of 43 food items in the month preceding hair collection. The established reference ranges were in line with data for other childhood or adolescent populations. The retained RRR factors explained 40, 50, 45, 46, 44 and 48 % of the variation of Ca, Cu, Fe, Mg, P and Zn concentrations in hair, respectively. Although this study demonstrated that a large proportion of hair mineral variation may be influenced by food consumption frequencies in elementary school girls, a number of food groups known to be rich sources of minerals did not show a relation with certain hair minerals. Future research should focus on mechanisms and processes involved in mineral incorporation and accumulation in scalp hair, in order to fully understand the importance and influence of diet on hair minerals.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid22935036,

title = {Direct trace-elemental analysis of urine samples by laser ablation-inductively coupled plasma mass spectrometry after sample deposition on clinical filter papers},

author = {Maite Aramendía and Luis Rello and Frank Vanhaecke and Martín Resano},

doi = {10.1021/ac3018839},

issn = {1520-6882},

year  = {2012},

date = {2012-10-01},

journal = {Anal Chem},

volume = {84},

number = {20},

pages = {8682--8690},

abstract = {Collection of biological fluids on clinical filter papers shows important advantages from a logistic point of view, although analysis of these specimens is far from straightforward. Concerning urine analysis, and particularly when direct trace elemental analysis by laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) is aimed at, several problems arise, such as lack of sensitivity or different distribution of the analytes on the filter paper, rendering obtaining reliable quantitative results quite difficult. In this paper, a novel approach for urine collection is proposed, which circumvents many of these problems. This methodology consists on the use of precut filter paper discs where large amounts of sample can be retained upon a single deposition. This provides higher amounts of the target analytes and, thus, sufficient sensitivity, and allows addition of an adequate internal standard at the clinical lab prior to analysis, therefore making it suitable for a strategy based on unsupervised sample collection and ulterior analysis at referral centers. On the basis of this sampling methodology, an analytical method was developed for the direct determination of several elements in urine (Be, Bi, Cd, Co, Cu, Ni, Sb, Sn, Tl, Pb, and V) at the low μg L(-1) level by means of LA-ICPMS. The method developed provides good results in terms of accuracy and LODs (≤1 μg L(-1) for most of the analytes tested), with a precision in the range of 15%, fit-for-purpose for clinical control analysis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RESANO201224,

title = {Direct analysis of silica by means of solid sampling graphite furnace atomic absorption spectrometry},

author = {M. Resano and E. Mozas and C. Crespo and J. Pérez and E. García-Ruiz and M. A. Belarra},

url = {https://www.sciencedirect.com/science/article/pii/S0584854712000572},

doi = {https://doi.org/10.1016/j.sab.2012.03.005},

issn = {0584-8547},

year  = {2012},

date = {2012-01-01},

journal = {Spectrochimica Acta Part B: Atomic Spectroscopy},

volume = {71-72},

pages = {24-30},

abstract = {This paper reports on the use of solid sampling-graphite furnace atomic absorption spectrometry for the direct analysis of synthetic amorphous silica. In particular, determination of hazardous elements such As, Cd, Cr, Cu, Pb and Sb is investigated, as required by regulations of the food industry. The conclusion of the work is that, after proper optimization of the working conditions, paying particular attention to the atomization temperature and the use of proper modifiers (graphite powder, HNO3 or Pd), it is possible to develop suitable procedures that rely on the use of aqueous standard solutions to construct the calibration curves for all the elements investigated. The proposed method shows important benefits for the cost-effective analysis of such difficult samples in routine labs, permitting fast screening of those elements that are very rarely present in this type of sample, but also accurate quantification of those often found, while offering low limits of detection (always below 0.1mgg−1) that comply well with legal requirements, and precision levels that are fit for the purpose (approx. 6–9% R.S.D.).},

note = {COLLOQUIUM SPECTROSCOPICUM INTERNATIONALE XXXVII (CSI XXXVII)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{EVENS201280,

title = {The use of liposomes to differentiate between the effects of nickel accumulation and altered food quality in Daphnia magna exposed to dietary nickel},

author = {Roel Evens and Karel A. C. De Schamphelaere and Lieve Balcaen and Yingying Wang and Karen De Roy and Martin Resano and M. Flórez and Nico Boon and Frank Vanhaecke and Colin R. Janssen},

url = {https://www.sciencedirect.com/science/article/pii/S0166445X1100333X},

doi = {https://doi.org/10.1016/j.aquatox.2011.11.017},

issn = {0166-445X},

year  = {2012},

date = {2012-01-01},

journal = {Aquatic Toxicology},

volume = {109},

pages = {80-89},

abstract = {A potential drawback of traditional dietary metal toxicity studies is that it is difficult to distinguish between the direct toxicity of the metal and indirect effects caused by altered concentrations of essential nutrients in the metal-contaminated diet. In previous studies it has become clear that this can hamper the study of the real impact of dietary metal exposure and also complicates the analysis of the mechanisms of dietary metal toxicity in filter-feeding freshwater invertebrates like Daphnia magna. This problem has been partly circumvented by the production of liposomes, since these vectors are invulnerable to metal-induced food quality shifts and as such can be applied to study the mechanisms of dietary metal toxicity without the confounding effect of nutritional quality shifts. The aim of current study was to evaluate if there is relevance for dietary Ni toxicity under natural exposures, i.e., when D. magna is exposed to dietary Ni via living algae, and secondly, to quantify how nutritional quality shifts contribute to the toxic effects that are observed when algae are used as contaminated food vectors. For this aim, liposomes were prepared by the hydration of phosphatidylcholine in media containing 0 (control), 10, 50, 100 and 500mgNi/L. The liposome particles were then mixed with uncontaminated green algae in a 1/10 ratio (on a dry wt basis) to make up diets with constant nutrient quality and varying Ni contents (i.e., 1.2μgNi/gdry wt in the control and 18.7, 140.3, 165.0 and 501.6μgNi/gdry wt in the Ni-contaminated diet, respectively). A second food type was prepared on the basis of a 1/10 mixture (on a dry weight basis) of control liposomes and Ni-contaminated algae, representing a diet that differed in Ni content (i.e., 1.2, 26.8, 84.7, 262.3 and 742.7μgNi/gdry wt) and concentrations of essential nutrients (in terms of P and omega 3 poly-unsaturated fatty acids like eicosapentaenoic acid and α-linolenic acid). Both diets were then simultaneously fed to D. magna during a 21-day chronic bioassay, using reproduction, growth, survival, ingestion rate and Ni bioaccumulation as endpoints. Ni delivered by liposomes caused a significant inhibition of reproduction and growth when the metal accumulated to minimum levels of 11.9 and 20.0μgNi/gdry wt after 7 and 14 days, respectively. Using algae as Ni vector, similar effects of dietary Ni exposure occurred when algae had been pre-exposed to concentrations of at least 133μg/L of bioavailable Ni (i.e., Ni2+), which is similar to the reproductive EC50 of waterborne Ni exposure for D. magna (115μgNi2+/L). While this may have some consequences for predicting chronic Ni toxicity in this range of Ni concentrations with the biotic ligand model – which could be further improved by including the dietary toxicity pathway in this model, the occurrence of such high concentrations in the field is very rare. Hence, there seems to be very little environmental relevance for dietary Ni toxicity to D. magna. Finally, besides the direct effects of Ni there was no evidence that nutritional quality shifts could have affected daphnids’ growth, but it is very likely that the impairment of reproduction at toxic exposure levels of Ni was also partly the result of reduced fatty acid levels.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{C2JA10322B,

title = {Direct determination of sulfur in solid samples by means of high-resolution continuum source graphite furnace molecular absorption spectrometry using palladium nanoparticles as chemical modifier},

author = {Martín Resano and María R. Flórez},

url = {http://dx.doi.org/10.1039/C2JA10322B},

doi = {10.1039/C2JA10322B},

year  = {2012},

date = {2012-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {27},

issue = {3},

pages = {401-412},

publisher = {The Royal Society of Chemistry},

abstract = {This work investigates the potential benefits of using Pd nanoparticles, in combination with Ru as permanent modifier, for sulfur monitoring as CS by means of high-resolution continuum source graphite furnace molecular absorption spectrometry. Upon heating, these small (approx. 20 nm diameter) Pd nanoparticles are evenly distributed over the platform surface, offering a larger surface for interaction with the analyte during the drying and pyrolysis steps. In this way, a more efficient stabilization of sulfur species can be achieved. Furthermore, a similar analytical response is obtained regardless of the chemical form in which sulfur species are originally found, thus making it easier to develop quantitative analytical methods for this analyte. When using these modifiers, and under optimized working conditions, it is possible to use this technique for direct analysis of different types of solid samples (biological, petroleum coke, polyethylene and steel CRMs), thus circumventing the traditional drawbacks associated with sample digestion when sulfur determination is aimed at. Accurate results are obtained using only the central CS line found at 257.958 nm, with precision values in the 5–10% RSD range (14 ng characteristic mass; 9 ng limit of detection). Moreover, the combined use of the main six CS lines available in the spectral area simultaneously monitored by the detector permits to further improve precision to 3–5% RSD, while decreasing the limit of detection down to 3 ng (the characteristic mass is also 3 ng), which represents a relative limit of detection of approx. 1 μg g−1, as calculated for the sample with the lowest sulfur content. In all cases, straightforward calibration with aqueous standards was proved to be feasible, and the sample throughput is of 3–4 samples per hour (5 replicates per sample).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{C1JA10193E,

title = {Laser ablation-inductively coupled plasma-mass spectrometry using a double-focusing sector field mass spectrometer of Mattauch–Herzog geometry and an array detector for the determination of platinum group metals and gold in NiS buttons obtained by fire assay of platiniferous ores},

author = {Martín Resano and Keith S. McIntosh and Frank Vanhaecke},

url = {http://dx.doi.org/10.1039/C1JA10193E},

doi = {10.1039/C1JA10193E},

year  = {2012},

date = {2012-01-01},

journal = {J. Anal. At. Spectrom.},

volume = {27},

issue = {1},

pages = {165-173},

publisher = {The Royal Society of Chemistry},

abstract = {This paper explores the potential of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) for the determination of Au, Ir, Pd, Pt, Rh and Ru in NiS buttons obtained by fire assay of platiniferous ores using a new type of ICP-MS instrument equipped with an array detector Mattauch–Herzog spectrometer. The method evaluated comprises the NiS fire assay of a representative amount of the ore sample (40–75 g), grinding of the NiS buttons subsequently obtained, pelleting of the resulting powders using polyethylene wax as a binder, and LA-ICP-MS analysis of the sample using in-house matrix-matched standards for calibration. The use of this new ICP-MS device proved very beneficial in this context, offering a remarkable level of precision (2–3% RSD for the most abundant analytes, Pt, Pd, Rh and Ru, and 6–11% RSD for Au and Ir) owing to its simultaneous capabilities and its extended linear range, which enabled an improved performance of the internal standard (61Ni). Moreover, the low level of argide-based interferences and the detection power of the instrument provided low limits of detection (10 ng g−1 level) even for those elements that could be potentially affected by ArNi+ and ArCu+ overlap in this particular matrix (Pd, Rh, Ru). The accuracy finally obtained (90% of results within 10% of the reference value, and 70% of results within 5%) is fit-for-purpose for this application. The resulting method can be thus considered very attractive as a faster and greener strategy for the control of these types of samples in the platinum group metal industry, circumventing the need for the cumbersome and environmentally unfriendly digestion procedures currently employed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}