
Call: +34 876 553 510
Email: ebolea@unizar.es
Address: c/Pedro Cerbuna 12, Universidad de Zaragoza, Facultad de Ciencias, Departamento de Química Analítica – Zaragoza (Spain)
ABOUT ME
Eduardo Bolea Fernández obtained his Bachelor and Master degrees in chemistry from the University of Zaragoza, Spain. He carried out PhD research at Ghent University, Belgium, and obtained his PhD degree in 2017. His PhD research focused on method development for ultra-trace elemental and isotopic analysis using tandem ICP-mass spectrometry (ICP-MS/MS). In October 2017, he got a postdoctoral research grant (BOF-UGent) focusing on high-precision isotopic analysis of mercury using multi-collector ICP-mass spectrometry for unravelling its biogeochemical cycle. In April 2018, he won the 2018 IUPAC-Solvay International Award for Young Chemists for the best PhD dissertation in the chemical sciences worldwide. In November 2019, he started a junior postdoctoral research grant (FWO) based on the development of new analytical methods and their application to metallomics and nanotechnology. In January 2022, he won the prestigious 2022 Young Scientist Winter Conference Award in Plasma Spectrochemistry for his contributions to the field of plasma spectrochemistry. In November 2022, he started a senior postdoctoral research grant (FWO) focusing on single-cell analysis in the biological sciences. In January 2023, he was awarded a 5-years Ramón y Cajal contract (Ministry of Science and Innovation, Spanish Government).
So far, Eduardo is (co)author of 39 publications in peer-reviewed international journals and his work has been presented in >50 lectures on international conferences and workshops.
PUBLICATIONS
2025
Bazo, Antonio; Bolea-Fernandez, Eduardo; Rua-Ibarz, Ana; Aramendía, Maite; Resano, Martín
En: Anal. Chem., 2025, ISSN: 1520-6882.
@article{nokey,
title = {Ions with Ions, Entities with Entities: A Proof-of-Concept Study Using the SELM-1 Yeast Certified Reference Material for Intra- and Extracellular Se Quantification via Single-Cell ICP-Mass Spectrometry},
author = {Antonio Bazo and Eduardo Bolea-Fernandez and Ana Rua-Ibarz and Maite Aramendía and Martín Resano},
url = {https://pubs.acs.org/doi/10.1021/acs.analchem.5c01588},
doi = {https://doi.org/10.1021/acs.analchem.5c01588},
issn = {1520-6882},
year = {2025},
date = {2025-06-07},
urldate = {2025-06-07},
journal = {Anal. Chem.},
abstract = {In this work, two novel nanoparticle (NP)-based calibration strategies, external calibration and a relative method, have been explored for single-cell ICP-mass spectrometry (SC-ICP-MS) analysis. The fundamental principle of these methods is to rely on individual entities (well-characterized NPs of the target analyte) for calibration rather than on ionic standard solutions. The performance of the NP-based calibration approaches has been compared to that of the reference method (particle size with AuNP standards). In addition to the intracellular Se content (mass per individual cell), the extracellular Se (dissolved fraction) was also determined directly and simultaneously using the average background from the SC-ICP-MS time-resolved signal. The figures-of-merit of the methods developed have been evaluated by relying on the analysis of the SELM-1 cell-certified reference material, consisting of Se-enriched yeast cells, and certified for its total Se content (intracellular + extracellular Se). All methods successfully determined the Se elemental contents, but an improvement in accuracy and precision was observed for the NP-based methods compared to the reference one. Furthermore, the NP-based methods were found to be less time-consuming, more straightforward, and more user-friendly in terms of calculations. These results open new avenues for calibration in quantitative SC-ICP-MS analysis and call for a fundamental change in the methodology, where the determination of ionic contents is based on the use of ionic standard solutions for calibration, while the determination of elemental contents in discrete micro/nanoentities, such as cells, should ideally be based on calibration using standard entities, thus avoiding the need to calculate a transport efficiency coefficient.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Rodler-Rørbo, Alexandra; Baragona, Anthony J.; Verbeemen, Eliah J.; Sørensen, Lasse Vilien; Çakmakoğlu, Berk; Helvaci, Cahit; Bolea-Fernandez, Eduardo; Rua-Ibarz, Ana; Vanhaecke, Frank; Becker, Hilary; Artioli, Gilberto; Zabrana, Lilli; Debaille, Vinciane; Mattielli, Nadine; Goderis, Steven; Claeys, Philippe
Cinnabar for Roman Ephesus: Material quality, processing and provenance Journal Article
En: Journal of Archaeological Science, vol. 173, pp. 106122, 2025, ISSN: 0305-4403.
@article{RODLERRORBO2025106122,
title = {Cinnabar for Roman Ephesus: Material quality, processing and provenance},
author = {Alexandra Rodler-Rørbo and Anthony J. Baragona and Eliah J. Verbeemen and Lasse Vilien Sørensen and Berk Çakmakoğlu and Cahit Helvaci and Eduardo Bolea-Fernandez and Ana Rua-Ibarz and Frank Vanhaecke and Hilary Becker and Gilberto Artioli and Lilli Zabrana and Vinciane Debaille and Nadine Mattielli and Steven Goderis and Philippe Claeys},
url = {https://www.sciencedirect.com/science/article/pii/S0305440324001900},
doi = {https://doi.org/10.1016/j.jas.2024.106122},
issn = {0305-4403},
year = {2025},
date = {2025-01-01},
journal = {Journal of Archaeological Science},
volume = {173},
pages = {106122},
abstract = {Ephesus was an important harbor city that flourished during the Roman period and ancient texts mention Almadén in Spain and the Cilbian fields of Ephesus as important cinnabar sources in antiquity. This work investigates whether imported cinnabar was used and whether this could be related to changes in painting activities over time. Microscopic analysis indicates a consistent preparation of cinnabar, hinting at a uniform source material quality or processing technique. However, the use of cinnabar varies among the architectural structures studied, indicating a plurality of painting techniques. A few of the analyzed cinnabar samples overlap with Turkish- and Balkan reference Pb isotope ratios; three samples from tabernas, however, deviate from this. The Hg isotope ratios reveal that cinnabar from carbonate-hosted deposits was likely used, and that processing of cinnabar included heating as suggested by ancient texts. Most notably, a correlation exists between the geochemical data and the painting technique – shifts in sourcing and cinnabar usage are potentially assignable to building chronology and/or usage. Through the lens of material provenance and processing, Ephesian cinnabar brings the organization of pigment trade into focus.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Sullivan, Kaj V.; Moser, Katharina; Costas-Rodríguez, Marta; Bolea-Fernandez, Eduardo; Vanhaecke, Frank
High-precision Cu isotopic analysis of human dietary Cu sources via multi-collector ICP-mass spectrometry Journal Article
En: Food Chemistry, vol. 470, pp. 142673, 2025, ISSN: 0308-8146.
@article{SULLIVAN2025142673,
title = {High-precision Cu isotopic analysis of human dietary Cu sources via multi-collector ICP-mass spectrometry},
author = {Kaj V. Sullivan and Katharina Moser and Marta Costas-Rodríguez and Eduardo Bolea-Fernandez and Frank Vanhaecke},
url = {https://www.sciencedirect.com/science/article/pii/S0308814624043231},
doi = {https://doi.org/10.1016/j.foodchem.2024.142673},
issn = {0308-8146},
year = {2025},
date = {2025-01-01},
journal = {Food Chemistry},
volume = {470},
pages = {142673},
abstract = {The disruption of Cu homeostasis is associated with the pathogenesis of many diseases and can result in alterations in Cu isotope fractionation. Changes in the Cu isotope ratio (65Cu/63Cu) of body fluids and tissues have been observed in liver disorders, cancers, and other diseases, displaying diagnostic/prognostic potential. However, it is not entirely clear whether certain physiological or lifestyle factors may also influence the bodily Cu isotopic composition, potentially obfuscating the signature of the pathology. To ascertain whether differences exist between food products, the Cu isotopic composition of 29 significant dietary Cu sources has been determined for the first time. The general enrichment of Cu and its heavy isotope, 65Cu, in legumes, nuts, and seeds (major Cu sources in plant-based diets) was revealed, suggesting that individuals with plant-based diets may have a bodily Cu isotopic composition offset from that of individuals with omnivorous diets, thus requiring controlling for in study design.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2024
Reis, Angélica S.; Paltian, Jaini J.; Domingues, William B.; Novo, Diogo L. R.; Bolea-Fernandez, Eduardo; Acker, Thibaut Van; Campos, Vinicius F.; Luchese, Cristiane; Vanhaecke, Frank; Mesko, Marcia F.; Wilhelm, Ethel A.
Platinum Deposition in the Central Nervous System: A Novel Insight into Oxaliplatin-induced Peripheral Neuropathy in Young and Old Mice Journal Article
En: Mol Neurobiol, 2024, ISSN: 1559-1182.
@article{Reis2024,
title = {Platinum Deposition in the Central Nervous System: A Novel Insight into Oxaliplatin-induced Peripheral Neuropathy in Young and Old Mice},
author = {Angélica S. Reis and Jaini J. Paltian and William B. Domingues and Diogo L. R. Novo and Eduardo Bolea-Fernandez and Thibaut Van Acker and Vinicius F. Campos and Cristiane Luchese and Frank Vanhaecke and Marcia F. Mesko and Ethel A. Wilhelm},
doi = {10.1007/s12035-024-04430-y},
issn = {1559-1182},
year = {2024},
date = {2024-09-25},
journal = {Mol Neurobiol},
publisher = {Springer Science and Business Media LLC},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Suárez-Criado, Laura; Bolea-Fernandez, Eduardo; Abou-Zeid, Lana; Vandermeiren, Mathias; Rodríguez-González, Pablo; Alonso, Jose Ignacio Garcia; Vanhaecke, Frank
En: J. Anal. At. Spectrom., vol. 39, iss. 2, pp. 592-600, 2024.
@article{D3JA00414G,
title = {Extending the application range of Hg isotopic analysis to sub-μg L−1 levels using cold vapor generation multi-collector inductively coupled plasma-mass spectrometry with 1013 ohm Faraday cup amplifiers},
author = {Laura Suárez-Criado and Eduardo Bolea-Fernandez and Lana Abou-Zeid and Mathias Vandermeiren and Pablo Rodríguez-González and Jose Ignacio Garcia Alonso and Frank Vanhaecke},
url = {http://dx.doi.org/10.1039/D3JA00414G},
doi = {10.1039/D3JA00414G},
year = {2024},
date = {2024-01-01},
urldate = {2024-01-01},
journal = {J. Anal. At. Spectrom.},
volume = {39},
issue = {2},
pages = {592-600},
publisher = {The Royal Society of Chemistry},
abstract = {High-precision determination of the isotopic composition of mercury (Hg) is of paramount importance for unraveling its biogeochemical cycle and for identifying the origin of Hg in environmental compartments. Cold vapor generation multi-collector inductively coupled plasma-mass spectrometry (CVG-MC-ICP-MS) is the standard approach for such application. Cold vapor generation provides a high Hg introduction efficiency into the ICP, while chromatographic Hg isolation is not required as a result of the selective reaction between Hg2+ and SnCl2. For environmental or biota samples with low Hg concentrations, however, this approach still presents challenges and reliable measurements typically require a Hg concentration ≥1 μg L−1 in the solution analyzed. Recent improvements of MC-ICP-MS instrumentation, including the introduction of the so-called Jet interface and 1013 Ω Faraday cup amplifiers, enhance the signal-to-noise ratio. In this study, it was investigated to what extent this allows Hg isotopic analysis at lower concentration. Performance in Hg isotopic analysis was compared using two different sets of cones (standard vs. Jet), two plasma conditions (wet vs. dry) and two amplifier types (1011 Ω vs. 1013 Ω). Satisfactory accuracy and precision were achieved at a Hg concentration down to 0.1 μg L−1 in the solution measured when using Jet cones, dry plasma conditions, and the four available 1013 Ω amplifiers. The uncertainty expressed as 2SD for the δ202Hg values measured for the in-house standard solution was ±0.2‰ at 0.25 μg Hg L−1 and ± 0.3‰ at 0.1 μg Hg L−1. The method was subsequently applied to the analysis of real surface water samples contaminated with toxic metals.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}