• Start date: 01-09-2023
  • End date: 31-08-2025
  • Coordinator: Martín Resano
  • Type: Proyectos de Generación de Conocimiento, Ministerio de Ciencia e Innovación

Summary: This project aims at developing new analytical methodologies for providing elemental and isotopic information from microsamples, which can be obtained in a minimally invasive way (e.g., dried matrix spots, DMSs), as well as from microentities (such as cells, nanoparticles, and micro/nanoplastics), in order to achieve entity-resolved information.

The methods developed should be simple and selective, requiring none or else minimal sample preparation (without digestions), since such methods avoid species interconversion, minimize risks of losses or contamination, and provide better limits of detection while ensuring a high sample throughput.

The hypothesis of the project is that atomic spectrometry techniques have experienced recent and significant improvements (e.g., faster acquisition capabilities, more potential to cope with spectral overlaps and capacities for new couplings, such as LA-ICP-MS/LIBS in tandem) that, together with recent advances in the production of DMSs of a constant and known volume, make it feasible to take microsampling in the biomedical field to the next level.

Thus, in this project, the most powerful trace elemental techniques (ICP-MS/MS, LA-ICP-MS/LIBS, and HR CS GFAAS) will be deployed, using state-of-the-art instrumentation, so it becomes therefore feasible to attain the specific goals pursued.

Key applications of interest in the biomedical field have been selected and will be investigated. The main focus of the project, however, will consist in building new approaches with a solid theoretical foundation that permit the acquisition of as much information as possible from minimal sample amounts, while enabling proper quantification.

The project will be developed around three different objectives: i) development of novel analytical strategies based on new instrumental configurations and couplings for the direct elemental and isotopic analysis of biomedical microsamples; ii) development of novel analytical strategies for minimally invasive analysis; and iii) development of methods based on time and/or spatially resolved information for the individual analysis of micro/nano entities.

Grant PID2021-122455NB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”.



Aramendía, Maite; Souza, André L. M.; Nakadi, Flávio V.; Resano, Martín

Boron elemental and isotopic determination via the BF diatomic molecule using high-resolution continuum source graphite furnace molecular absorption spectrometry Journal Article

En: J. Anal. At. Spectrom., vol. 39, pp. 767-779, 2024.

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Suárez-Criado, Laura; Bolea-Fernandez, Eduardo; Abou-Zeid, Lana; Vandermeiren, Mathias; Rodríguez-González, Pablo; Alonso, Jose Ignacio Garcia; Vanhaecke, Frank

Extending the application range of Hg isotopic analysis to sub-μg L−1 levels using cold vapor generation multi-collector inductively coupled plasma-mass spectrometry with 1013 ohm Faraday cup amplifiers Journal Article

En: J. Anal. At. Spectrom., vol. 39, iss. 2, pp. 592-600, 2024.

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Rua-Ibarz, Ana; Acker, Thibaut Van; Bolea-Fernandez, Eduardo; Boccongelli, Marina; Vanhaecke, Frank

A comparison of calibration strategies for quantitative laser ablation ICP-mass spectrometry (LA-ICP-MS) analysis of fused catalyst samples Journal Article

En: J. Anal. At. Spectrom., vol. 39, iss. 3, pp. 888-899, 2024.

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Bolea-Fernandez, Eduardo; Rua-Ibarz, Ana; Anjos, Jorge Alves; Vanhaecke, Frank

Development and initial evaluation of a combustion-based sample introduction system for direct isotopic analysis of mercury in solid samples via multi-collector ICP-mass spectrometry Journal Article

En: Talanta, vol. 276, pp. 126210, 2024, ISSN: 0039-9140.

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Acker, Thibaut Van; Rua-Ibarz, Ana; Vanhaecke, Frank; Bolea-Fernandez, Eduardo

Laser Ablation for Nondestructive Sampling of Microplastics in Single-Particle ICP-Mass Spectrometry Journal Article

En: Anal. Chem., vol. 95, iss. 50, pp. 18579-18586, 2023.

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Sullivan, Kaj Vaughan; Assantuh, Yasmina; Grigoryan, Rosa; Costas-Rodríguez, Marta; Bolea-Fernandez, Eduardo; Lapauw, Bruno; Laecke, Steven Van; Vanhaecke, Frank

Serum Mg Isotopic Composition Reveals That Mg Dyshomeostasis Remains in Type 1 Diabetes despite the Resolution of Hypomagnesemia Journal Article

En: Int. J. Mol. Sci., vol. 24, iss. 21, pp. 15683, 2023.

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Aramendía, Maite; Leite, Diego; Resano, Javier; Resano, Martín; Billimoria, Kharmen; Goenaga-Infante, Heidi

Isotope Dilution Analysis for Particle Mass Determination Using Single-Particle Inductively Coupled Plasma Time-of-Flight Mass Spectrometry: Application to Size Determination of Silver Nanoparticles Journal Article

En: Nanomaterials, vol. 13, iss. 17, pp. 2392, 2023.

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Bazo, Antonio; Aramendía, Maite; Nakadi, Flávio V.; Resano, Martín

An Approach Based on an Increased Bandpass for Enabling the Use of Internal Standards in Single Particle ICP-MS: Application to AuNPs Characterization Journal Article

En: Nanomaterials, vol. 13, no. 12, 2023, ISSN: 2079-4991.

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García-Poyo, M. Carmen; Bérail, Sylvain; Ronzani, Anne Laure; Rello, Luis; García-González, Elena; Nakadi, Flávio V.; Aramendía, Maite; Resano, Javier; Resano, Martín; Pécheyran, Christophe

Cu fractionation, isotopic analysis, and data processing via machine learning: new approaches for the diagnosis and follow up of Wilson's disease via ICP-MS Journal Article

En: J. Anal. At. Spectrom., vol. 38, iss. 1, pp. 229-242, 2023.

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Bazo, Antonio; Garde, Raúl; Garcia-Ruiz, Esperanza; Aramendía, Maite; Nakadi, Flávio V.; Resano, Martín

High-resolution continuum source graphite furnace molecular absorption spectrometry for the monitoring of Sr isotopes via SrF formation: a case study Journal Article

En: J. Anal. At. Spectrom., vol. 37, iss. 12, pp. 2517-2528, 2022.

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